Micropipette Technique
Micropipette Technique - Related Videos
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
07:02
Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing
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Cited by 1 •
2024
The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.
02:44
Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models
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Cited by 6 •
2024
Here, we describe micropipette-guided drug administration (MDA) as an alternative method to oral gavage that incentivizes the research animal to ingest treatments readily with minimal stress and discomfort.
09:29
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
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Cited by 6 •
2020
The goal of the protocol is to reliably measure membrane mechanical properties of giant vesicles by micropipette aspiration.
09:54
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Cited by 8 •
2015
Bacterial mechanosensitive channels can be used as mechanoelectrical transducers in biomolecular devices. Droplet interface bilayers (DIBs), cell-inspired building blocks to such devices, represent new platforms to incorporate and stimulate mechanosensitive channels. Here, we demonstrate a new micropipette-based method of forming DIBs, allowing the study of mechanosensitive channels under mechanical stimulation.
FAQs
In an adjustable air-displacement micropipette, the spring-loaded plunger moves a piston that changes the volume of air above the disposable tip. Releasing the plunger draws liquid into the tip, while pressing it dispenses the liquid. Selecting the intended volume determines how much air is displaced and therefore the transferred amount.
Tip attachment, immersion depth, and plunger speed directly influence measurement quality. A securely attached tip supports consistent liquid handling, while placing it at an appropriate depth helps the instrument draw the intended sample without avoidable variation. Smooth, steady plunger control improves dispensing consistency, which is especially important when biological samples must be treated alike.
Repeatedly using controlled handling reduces measurement error and helps limit contamination, so differences among biological samples are more likely to reflect the experiment rather than transfer inconsistencies. This reliability supports reproducible solution preparation, sample transfers, and reaction assembly, allowing results from separate measurements or experiments to be compared more confidently.
Before transferring a sample, select the required volume, attach the disposable tip securely, and control the spring-loaded plunger smoothly. Use the intended immersion depth while drawing the liquid, then dispense it with steady control into the receiving vessel. Following the same sequence for each transfer promotes consistent volumes and reduces handling-related error.
A micropipette transfers measured portions from one solution to the next so each step can produce a progressively diluted sample. Accurate volume selection and consistent plunger handling are essential because an error introduced early can affect later dilution steps. This makes careful technique important in biological assays and solution preparation.
Micropipette technique supports solution preparation, sample transfer, serial dilution, and reaction assembly across molecular biology, microbiology, cell culture, and biochemical analysis. In each setting, controlled liquid handling helps researchers combine or move biological materials in reproducible amounts. Reliable transfers strengthen experimental consistency and help reduce errors that could affect downstream results.