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1Department of Mechanical Engineering,Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences,Virginia Polytechnic Institute and State University, 3Department of Biology,University of Maryland, 4College of Engineering,University of Georgia, 5Department of Engineering Sciences and Mechanics,Virginia Polytechnic Institute and State University
Chapters
- 00:05Title
- 00:48Preparation of Liposomes
- 02:00Manufacture of the Oil Reservoir and Preparation of Electrodes
- 04:06Setting Up the Experiment
- 06:22Formation of the Lipid Bilayer
- 07:49Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli
- 08:59Conclusion
Bacterial mechanosensitive channels can be used as mechanoelectrical transducers in biomolecular devices. Droplet interface bilayers (DIBs), cell-inspired building blocks to such devices, represent new platforms to incorporate and stimulate mechanosensitive channels. Here, we demonstrate a new micropipette-based method of forming DIBs, allowing the study of mechanosensitive channels under mechanical stimulation.
