Mouse Embryo Fibroblasts
Mouse Embryo Fibroblasts - Related Videos
02:52
Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse
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2025
This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.
09:38
Direct Reprogramming of Mouse Fibroblasts into Melanocytes
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Cited by 2 •
2021
Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.
12:59
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Cited by 1 •
2017
This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage.
06:35
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
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Cited by 95 •
2016
We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.
09:57
Dissection of 6.5 dpc Mouse Embryos
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Cited by 28 •
2007
Isolation of postimplantation-stage embryos allows one to study gene patterning and analyze cell-lineage decision making processes during embryonic development, but proper dissection of the early embryo can be challenging. This protocol describes a method for isolating early primitive-streak-stage embryos (~6.5 days post coitum [dpc]).
FAQs
After tissue dissociation, the cells attach to the culture surface and proliferate in nutrient medium. They also produce extracellular-matrix components and signaling factors, which can alter the surrounding culture environment. These activities make MEFs useful not only as growing cells, but also as a source of structural and biochemical support for other cells maintained in vitro.
Mitotic inactivation stops MEFs from dividing while preserving their ability to provide feeder-cell support. This separates two functions that could otherwise interfere: the cells can create a supportive environment without continuing to expand as a competing population. The approach is therefore useful when researchers need stable culture support for embryonic stem cells or related experimental systems.
Their defined developmental origin gives researchers a consistent biological context for examining cellular physiology and experimental disease models. At the same time, MEFs can be manipulated in culture, allowing investigators to study growth, senescence, and genetic effects under controlled conditions. This combination of developmental specificity and experimental flexibility supports comparisons across cell-based studies.
Preparation begins with developing mouse embryonic tissue, which is dissociated to release fibroblasts. The resulting cells are placed in nutrient medium and allowed to adhere to a culture surface, where they can proliferate. Depending on the planned use, the cultured population may then be mitotically inactivated to provide support without continued cell division.
MEFs are used when embryonic stem cells require a supportive feeder-cell environment or when induced-pluripotent-stem-cell reprogramming needs a conditioned culture setting. Their extracellular-matrix production and signaling factors help establish that environment. Researchers can use actively growing or mitotically inactivated cells according to whether they need proliferating fibroblasts or stable feeder support.
MEF cultures support studies of cell growth, cellular senescence, genetic effects, and experimental disease models. They can also generate conditioned environments for examining how fibroblast-derived factors influence other cells. Because researchers can culture and manipulate them in vitro, MEFs connect defined developmental material with controlled investigations of cellular physiology and disease-related processes.