Oxygen-rich Environment
Oxygen-rich Environment - Related Videos
10:15
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
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2019
We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.
06:08
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
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Cited by 13 •
2018
Here, we present a method of the spectroscopic characterization of organic molecules by means of time-resolved photoluminescence spectroscopy on the nanosecond-to-millisecond timescale in oxygen-free conditions. Methods to efficiently remove oxygen from the samples and, thus, limit luminescence quenching are also described.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 27 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
09:38
Dissolved Oxygen in Surface Water
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Dissolved oxygen (DO) measurements calculate the amount of gaseous oxygen dissolved in surface water, which is important to all oxygen-breathing life in river ecosystems, including fish species preferred for human consumption (e.g. bluegill and bass), as well as decomposer species critical to the recycling of biogeochemical materials in the system. The oxygen dissolved in lakes, rivers, and oceans is crucial for the...
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
Greater oxygen availability can support diffusion into tissues and provide mitochondria with more of the terminal electron acceptor required for aerobic respiration. This pathway supports ATP production, so oxygen-rich conditions can alter cellular energy metabolism. The outcome is not simply increased energy generation, because the same conditions may also intensify oxidative stress and trigger protective cellular responses.
Mitochondrial use of oxygen for aerobic respiration can be accompanied by increased production of reactive oxygen species, chemically reactive molecules that can influence cellular function. Cells respond by activating antioxidant defenses, changing gene expression, and adjusting metabolism. These responses help explain why elevated oxygen may affect cellular survival as well as energy production.
Changes may occur in several connected processes, including aerobic metabolism, antioxidant defense, gene expression, and cellular survival. Increased oxygen availability can modify how cells generate energy, while reactive oxygen species can act as a stress signal that prompts regulatory responses. Studying these linked changes helps distinguish direct metabolic effects from broader cellular adaptation.
Investigators examine oxygen-rich conditions in cell culture, developmental research, environmental physiology, and disease models. Depending on the system, they assess effects on metabolism, oxidative stress, tissue responses, or adaptation. Comparing biological responses under elevated oxygen with those associated with typical ambient air can reveal how oxygen availability influences cells, tissues, organisms, and disease-related processes.
Cell-culture studies can isolate how elevated oxygen affects cellular metabolism, oxidative stress, gene expression, and survival. They provide a controlled biological setting for examining responses that may be difficult to separate in whole organisms. Results can help clarify whether cells adjust their energy production, strengthen antioxidant defenses, or show stress-related changes under oxygen-rich conditions.
Developmental studies use oxygen-rich conditions to examine how changing oxygen availability influences tissue responses and biological adaptation. Disease models apply the same principle to investigate altered metabolism, oxidative stress, and cellular survival in disease-related settings. Together, these applications connect oxygen-dependent mechanisms with tissue behavior and help identify how biological systems respond to elevated oxygen.