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Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
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Biochemistry
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JoVE Journal Biochemistry
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

DOI:
10.3791/58727-v

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10:15 min

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January 07, 2019

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, ,
1Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, 2Université Grenoble Alpes, 3Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Chapters

  • 00:04Title
  • 00:40Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
  • 02:34Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
  • 04:32Lysis and In-Gel Digestion
  • 07:54Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.

Tags

Keywords: Proteomic AnalysisHuman Macrophage PolarizationLow Oxygen EnvironmentLabel-free QuantificationPBMC IsolationCD14+ Monocyte IsolationMacrophage Differentiation

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