Polyethyleneimine Pei
Polyethyleneimine Pei - Related Videos
09:36
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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Cited by 21 •
2019
We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.
06:49
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
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2022
Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.
09:09
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Cited by 10 •
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In this protocol, doxorubicin-loaded AS1411-g-PEI-g-PEG modified gold nanoparticles are synthesized via three-step amide reactions. Then, doxorubicin is loaded and delivered to target cancer cells for cancer therapy.
06:30
Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
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Cited by 2 •
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Here, we present a protocol for a mosaic labeling technique that permits the visualization of neurons derived from a common progenitor cell in two distinct colors. This facilitates neural lineage analysis with the capability of birth-dating individual neurons and studying gene function in the same neurons of different individuals.
05:50
Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and Functional Testing
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2026
This protocol presents Rayleigh breakup as a mild process for aerosolizing non-viral mRNA vectors. Regional deposition for aerosolized mRNA vectors is evaluated by the Alberta Idealized Nasal Inlet (AINI) model. Green fluorescence protein (GFP) expression in human lung cells represents maintenance of physicochemical and biological functions of post-aerosolized mRNA vectors.
FAQs
PEI’s protonatable amine groups provide positive charges that associate electrostatically with negatively charged DNA or RNA. This compaction produces polyplexes, which can shield nucleic acids during handling and delivery. After uptake, the same proton-buffering behavior within endosomes can assist release into the cell, linking chemical structure to transfection performance.
Performance depends on more than the presence of positive charge. PEI concentration, molecular weight, and structural features influence how effectively it complexes with nucleic acids and supports delivery, but stronger activity may also increase cytotoxicity. Optimization therefore requires balancing genetic-material transport against the cells’ tolerance of the polymer.
Because PEI binds negatively charged molecules, it can interact with both DNA and RNA rather than being limited to one nucleic-acid type. The same property also permits immobilization on biological material surfaces, extending its use from transient genetic delivery in cells to modifying biologically relevant interfaces.
A basic workflow starts by combining PEI with a DNA or RNA preparation so electrostatic polyplexes can form. The complexes are then used with cultured cells for gene transfection or delivery of a genetic construct. Researchers should vary concentration, molecular weight, or structure as needed, while observing whether delivery gains are accompanied by unacceptable cytotoxicity.
The relevant outcome is not transfection activity alone. A useful condition should promote delivery of the intended genetic material while preserving acceptable cellular tolerance, because the variables that improve activity can also increase cytotoxicity. This balance helps determine whether a PEI formulation is suitable for a particular cultured-cell experiment.
PEI can support surface modification by providing a positively charged interface that helps immobilize negatively charged molecules on biological materials. In this setting, the goal is not necessarily to transport genetic material into cells, but to associate selected molecules with a material surface, broadening its biological use beyond laboratory transfection.