Proximal Tubule Labeling
Proximal Tubule Labeling - Related Videos
07:06
Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule
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Cited by 3 •
2025
We present an optimized protocol for processing whole human kidneys to isolate and culture primary renal proximal tubule epithelial cells and the application of these cells in a three-dimensional, microfluidic, microphysiological platform to recapitulate the renal proximal tubule.
05:11
Malpighian Tubule Dissection from an Adult Fly: Isolating an Osmoregulatory and Excretory Organ
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2023
This article describes the dissection of the anterior Malpighian tubules (MT) from a female Drosophila fly and an example protocol from Rossano et al. (2017) in which the MTs are prepared for live imaging in a perfusion chamber.
04:25
Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
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2025
This video demonstrates the isolation procedure of Sertoli cells from mouse seminiferous tubules, a component of the testes. Enzymatic digestion and repeated washes promote the release of Sertoli cells into the solution, which can be cultured further for proliferation and growth.
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Cited by 6 •
2022
A neuronal lysosome proximity labeling proteomics protocol is described here to characterize the dynamic lysosomal microenvironment in human induced pluripotent stem cell-derived neurons. Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human neurons.
08:36
AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
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Cited by 1 •
2022
Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.
FAQs
Tracer-based approaches rely on the proximal tubule’s transport and endocytic pathways. A tracer enters cells through these routes and produces a detectable signal, allowing investigators to identify labeled tubule segments or cells. This mechanism connects the observed signal with the cellular handling of substances, making the approach useful for examining solute transport and reabsorption in renal biology.
Segment-specific molecular markers identify proximal tubule cells through characteristic molecular features, whereas tracers reveal cells or segments based on their uptake through transport or endocytic pathways. The two strategies therefore provide different kinds of information: markers support cellular identification, while tracers can relate labeling to transport-related behavior. Either approach can help distinguish the proximal tubule from other nephron regions.
The proximal tubule performs region-specific solute reabsorption and transport, so separating it from other nephron regions is essential when interpreting renal experiments. Labeling provides a way to associate microscopic or analytical signals with this particular segment. That distinction supports more precise studies of proximal tubule structure, function, cellular injury, and responses to experimental treatments.
The distribution of a label can help map proximal tubule structure and indicate which cells or segments are being examined. When the label enters through transport or endocytic pathways, its localization also provides context for investigating how proximal tubule cells handle solutes. These observations help connect cellular identity with renal transport processes and support analysis of segment-specific biology.
A study generally selects either a segment-specific molecular marker or a tracer suited to the biological question, allows the labeling strategy to identify proximal tubule cells or segments, and then examines the resulting detectable signal. Microscopy or another analytical method is used to visualize or analyze that signal, after which researchers interpret structure, transport, or cellular responses.
Researchers can apply this approach when they need to examine kidney development, characterize proximal tubule structure, investigate solute reabsorption or transport, or distinguish proximal tubule cells from other nephron regions. It is also relevant to studies of cellular injury, disease mechanisms, and responses to experimental treatments, because labeling links observed findings to a defined renal cell population or segment.
By identifying proximal tubule cells or segments, labeling helps researchers determine where structural or cellular changes occur within the nephron. The resulting signal can be examined with microscopy or other analytical methods alongside questions about transport, reabsorption, development, or treatment response. This localization provides context for interpreting injury patterns and disease-related changes in renal biology.