Q-tof Instrument
Q-tof Instrument - Related Videos
07:27
MALDI-TOF Mass Spectrometry
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2023
Matrix-assisted laser desorption ionization (MALDI) is a mass spectrometry ion source ideal for the analysis of biomolecules. Instead of ionizing compounds in the gaseous state, samples are embedded in a matrix, which is struck by a laser. The matrix absorbs the majority of the energy; some of this energy is then transferred to the sample, which ionizes as a result. Sample ions can then be identified using a time-of-flight analyzer (TOF). This video covers principles of MALDI-TOF, including...
06:16
MALDI-ToF MS Method for the Characterization of Synthetic Polymers with Varying Dispersity and End Groups
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2025
This protocol provides detailed instructions and examples for sample preparation, data acquisition, and data analysis to characterize synthetic polymers with varying dispersity and end-groups using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Cited by 29 •
2016
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.
09:48
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
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Cited by 13 •
2016
This work presents a protocol for liquid handling and sample introduction to a microchannel for in situ time-of-flight secondary ion mass spectrometry analysis of protein biomolecules in an aqueous solution.
01:25
Research Instruments
0 Views •
2024
Effective research relies on specialized instruments that help collect and analyze data precisely. These tools, which include surveys, interviews, observation checklists, focus groups, and mechanical devices, are each designed to capture specific types of information crucial for informed decision-making. Surveys, for instance, are commonly used to gather broad insights from large populations, such as determining public opinion on educational policies. Interviews, on the other hand, play a...
FAQs
The quadrupole transmits or selects ions according to their mass-to-charge ratios before they enter the later stages of analysis. This filtering step determines which ions undergo further examination and helps organize measurements from a complex molecular sample. By controlling the ions that proceed, the instrument can focus structural analysis on selected mass-to-charge signals.
Collision-induced dissociation breaks selected ions into smaller ions before time-of-flight separation. The resulting products provide structural information that cannot be obtained from molecular mass alone. Comparing the masses of the original and fragment ions helps characterize molecules, making this process especially valuable for peptide sequencing and biomolecule identification.
In the time-of-flight analyzer, ions are separated according to how quickly they reach the detector. Their arrival behavior is related to mass-to-charge ratio, allowing the instrument to distinguish ions and measure their molecular masses with high accuracy. This capability supports confident analysis when biological samples contain multiple molecules with closely related signals.
A typical workflow begins by transmitting or selecting ions with the quadrupole, followed by collision-induced dissociation of the selected ions. The resulting ions then pass through the time-of-flight analyzer, where their arrival times separate them for detection. Researchers interpret the measured mass-to-charge values and fragment information together to identify or characterize molecules.
The platform is suited to high-resolution proteomics, metabolomics, peptide sequencing, and biomolecule identification. These applications use accurate molecular-mass measurements to detect or characterize molecules and use fragment-ion information to investigate structure. Its combination of mass accuracy and structural data makes it useful for examining complex biological samples rather than relying on mass measurement alone.
Q-tof analysis can provide both accurate molecular-mass measurements and structural information from the same analytical process. Mass-to-charge signals help distinguish and identify molecular components, while ions produced by collision-induced dissociation contribute evidence about molecular structure. In biology, this combined information supports interpretation of peptides, metabolites, proteins, and other biomolecules in complex samples.