Real-time Fluorescence Feedback
Real-time Fluorescence Feedback - Related Videos
08:16
Movement Retraining using Real-time Feedback of Performance
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Cited by 4 •
2013
Retraining abnormal movement patterns following injury or disease is a key component of physical rehabilitation. Recent advances in technology have permitted accurate assessment of movement during a variety of tasks, with near instantaneous quantification of results. This provides new opportunities for modification of faulty movement patterns in real time.
07:05
A Protocol for the Administration of Real-Time fMRI Neurofeedback Training
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Cited by 5 •
2017
The ability to induce and/or control neural plasticity may be critical in future treatments for neurologic disorders and the recovery from brain injury. In this paper, we present a protocol on the use of neurofeedback training with functional magnetic resonance imaging to modulate human brain function.
06:37
Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
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Cited by 41 •
2010
Fluorescence imaging is a promising innovative modality for image-guided surgery in surgical oncology. In this video we describe the technical procedure for detection of the sentinel lymph node using fluorescence imaging as showcased in gynecologic oncologicy. A multispectral fluorescence camera system, together with the fluorescent agent indocyanine green, is applied.
12:23
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
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Cited by 6 •
2012
We demonstrate FRET between conjugated polymer polydiacetylene (PDA) and fluorophore attached to the surface of PDA liposomes for the sensing of biomolecules. PDA liposomes also contained receptor molecules on their surfaces for biomolecules to be used as probes. Ligand-receptor interactions lead to changes in the FRET efficiency between the fluorophore and PDA which is the basis of the sensing mechanism.
08:32
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
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Cited by 21 •
2017
This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.
FAQs
The system interprets changes in fluorescence intensity or other signal features as measurements of a changing biological process. Detectors capture these changes after reporter excitation, while software evaluates the incoming data and determines whether the observed pattern warrants a response. This converts fluorescence from a passive readout into information that can support timely experimental decisions.
Fluorescent reporters provide the measurable light signal, excitation initiates that emission, and detectors capture the resulting changes over time. The quality and meaning of the feedback depend on preserving this sequence: the reporter must reflect the biological process being studied, and the detector must record signal features that software can analyze. Together, these components connect biology with control.
Continuous monitoring can reveal short-lived changes that might be missed when measurements occur only after an experiment or at widely separated time points. Because software evaluates signals as the process unfolds, the system can respond while the event is still occurring. This is particularly relevant to live-cell experiments and other assays in which biological conditions change over time.
A workflow begins by monitoring a fluorescent reporter with excitation and detection, then sending the collected signal to software for analysis. The software evaluates intensity or other signal features and links the result to a predefined experimental decision, such as changing conditions or triggering an intervention. This sequence creates a measurement-to-response loop that operates during the experiment.
The approach can support monitoring of cellular activity, gene expression, molecular interactions, and responses to environmental conditions. Its value differs with the biological question: fluorescence may track activity within living cells, indicate changes in expression, follow interaction-related signals, or show how a system responds to altered surroundings. The resulting time-resolved information helps relate biological changes to experimental conditions.
Researchers would choose it when the timing of a biological change matters or when experimental conditions may need adjustment during the assay. Linking measurement to control can reduce delays and improve precision in live-cell experiments and other time-dependent studies. It also helps researchers examine how cellular or molecular behavior develops rather than considering only a final outcome.