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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
JoVE Journal
Biology
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JoVE Journal Biology
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

DOI:
10.3791/55486-v

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08:32 min

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March 16, 2017

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, , , , , , , , , , , , ,
1Institute of Molecular Biology and Biochemistry,Medical University of Graz

Chapters

  • 00:05Title
  • 01:07Cell Preparation
  • 02:33Live Cell Imaging of NO• and Ca2+ Signals in Individual Cells
  • 06:28Results: Simultaneous Multichannel Imaging of NO• and Ca2+ Signals in Single EA.hy926 Cells
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

Tags

Keywords: Genetically Encoded Fluorescent Nitric Oxide ProbesGeNOpsReal-time ImagingNitric Oxide SignalsSingle CellsFluorescence MicroscopeIron(II) Booster SolutionFura-2AMPerfusion ChamberGravity-driven PerfusionPhysiological Calcium Buffer

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