S100b Protein Target
S100b Protein Target - Related Videos
05:22
Using Phage Display to Select Proteins with High Affinity to a Target Protein
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2025
In this video, we demonstrate the phage display technique to isolate pre-engineered phages expressing proteins with high affinity to the target protein.
03:52
Immunofluorescence Assay to Evaluate Effect of Target Proteins on Neurite Outgrowth
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2025
In this video, we demonstrate an EGFP-based immunofluorescence assay to identify the efficacy of target proteins on the neurite outgrowth of primary cortical neurons. The primary neurons are transfected with a fluorescent protein, EGFP, and a target protein, and then an immunoassay is performed to investigate the effect of the target protein on neurite outgrowth.
05:16
Assessing the Internalization of Target Surface Proteins Using a Biotin Derivative
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2025
This video demonstrates a method to assess target protein internalization in the mouse cortical astrocytes using biotinylation, followed by cell lysis, streptavidin-based protein extraction, denaturation, and Western blot analysis to confirm successful internalization.
04:39
Quantitative Dot Blot to Estimate Target Proteins in a Tissue Lysate
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2025
This video demonstrates a quantitative dot blot, or QDB, technique for the absolute quantification of proteins of interest in a tissue sample. The proteins in the sample are immobilized on the nitrocellulose membrane inside the wells of a QDB plate. Utilizing target protein-specific antibodies labeled with a peroxidase enzyme, a chemiluminescent substrate is oxidized to produce light, which is measured to determine the protein concentration of the sample.
06:25
Electrochemiluminescence Assay for Quantification of Target Protein Levels in Brain Lysate
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2025
In this video, we demonstrate the electrochemiluminescence immunoassay for the quantitation of target protein levels in different samples. This assay is based on the luminescence produced in the electrochemical reaction. The intensity of light emitted is proportional to the quantity of the target protein in the sample.
FAQs
Calcium binding to S100B’s EF-hand domains triggers a conformational change, meaning the protein adopts a different three-dimensional shape. This altered state enables S100B to interact with intracellular proteins and regulate cellular responses. The calcium-dependent structural switch is therefore central to studying how changes in calcium signaling may influence nervous-system biology.
Outside cells, S100B can engage the receptor for advanced glycation end products, or RAGE. This interaction connects extracellular S100B to downstream inflammatory and survival pathways, providing a mechanism through which glial stress or injury may influence surrounding cellular responses. Investigating this receptor interaction helps clarify S100B-related neuroinflammatory signaling.
S100B levels can provide an indication of glial stress or injury, while activity reflects how the protein participates in signaling interactions. Examining both aspects helps researchers distinguish the presence of a biological response from the mechanisms associated with it. This distinction is relevant when studying disease processes or evaluating targeted interventions.
Research commonly examines S100B levels, activity, and interactions with signaling partners in relation to glial stress, injury, or disease. Investigators can then assess whether observed changes align with neuroinflammation or brain injury and use the target to evaluate interventions. These approaches support both biomarker research and mechanistic studies of neurological conditions.
S100B measurements can reflect glial stress or injury, making the protein useful in neuroscience research focused on biological changes in the nervous system. Its signal may help researchers investigate brain injury, neuroinflammation, and neurological disease. However, the overview supports its use primarily for diagnostic research and evaluation, rather than treating a measurement as a complete explanation by itself.
S100B connects cellular stress with signaling pathways that may influence inflammation and survival. Researchers can therefore examine its levels and activity to relate glial responses to brain injury or neurological disease, while also investigating RAGE-linked mechanisms. This combination makes the target relevant for understanding disease biology and assessing whether interventions affect associated signaling responses.