Spio Nanoparticles
Spio Nanoparticles - Related Videos
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
07:40
Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
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Cited by 14 •
2017
The antimicrobial properties of metals such as copper and silver have been recognized for centuries. This protocol describes pulsed laser-ablation in liquids, a method of synthesizing metal nanoparticles that provides the ability to fine tune the properties of these nanoparticles to optimize their antimicrobial effects.
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
14:04
Viral Nanoparticles for In vivo Tumor Imaging
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Cited by 45 •
2012
Plant viral nanoparticles (VNPs) are promising platforms for applications in biomedicine. Here, we describe the procedures for plant VNP propagation, purification, characterization, and bioconjugation. Finally, we show the application of VNPs for tumor homing and imaging using a mouse xenograft model and fluorescence imaging.
FAQs
Their iron oxide cores disturb the relaxation behavior of nearby hydrogen protons after exposure to the MRI magnetic field. This local effect increases contrast most strongly in T2- and T2*-weighted images, allowing labeled cells or tissues to become more distinguishable from surrounding biological structures. The resulting signal changes support noninvasive visualization and localization.
Superparamagnetism allows SPIO nanoparticles to become strongly magnetized while an external magnetic field is applied, but retain little magnetization after the field is removed. This behavior supports magnetic responsiveness during imaging or separation without persistent magnetization between exposures. Consequently, the particles can act as controllable magnetic labels in biological systems.
Surface coatings improve the particles’ stability and dispersibility, helping them remain usable in biological environments. They also influence how the nanoparticles interact with cells, including cellular uptake. These properties are important because the coating can affect whether particles remain sufficiently distributed for labeling, tracking, or imaging rather than becoming poorly dispersed.
Cells can be associated with SPIO nanoparticles so that the iron oxide signal provides a magnetic imaging label. After labeling, researchers can follow the cells noninvasively as they move through or persist within tissues. This approach supports studies of cell location and behavior over time, including investigations of disease progression and therapeutic responses.
SPIO nanoparticles support tumor and organ imaging, magnetic separation, and investigations of biodistribution. These applications allow researchers to examine where labeled material or cells are located, how they distribute through biological systems, and how disease or treatment changes their behavior. Their combined imaging and magnetic properties make them useful across multiple experimental contexts.
By enabling noninvasive imaging of labeled cells and tissues, SPIO nanoparticles help researchers monitor biological changes without relying only on endpoint measurements. Imaging can be used alongside biodistribution studies to examine disease progression or therapeutic responses. In this context, the particles provide a way to connect spatial information with changes occurring during an experiment.