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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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1Biomedical Engineering Department,Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center,Johns Hopkins University School of Medicine, 3Wilmer Eye Institute,Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology,Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 01:21Cell Seeding and Transfection
- 04:34Nanoparticle Sizing and Plasmid per Particle Calculations
- 06:37Representative Data
- 08:01Conclusion
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
Tags
Keywords:
Polymeric Gene DeliveryNanoparticle Tracking AnalysisHigh-throughput Flow CytometryNon-viral Gene DeliveryCationic PolymersPolylysinePolyphosphoestersPoly(amidoamines)Polyethylenimine (PEI)Poly(beta-amino Ester)s (PBAEs)Human Retinal Endothelial Cells (HRECs)Transfection Efficiency96-well Plate Assay
