Stereo Microscope
Stereo Microscope - Related Videos
10:22
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
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2025
This article presents a protocol to perform comprehensive spatial transcriptomic profiling of host, viral, fungal, and microbiome RNA from formalin-fixed paraffin-embedded tissue sections using Stereo-seq, which enables high-resolution mapping of diverse transcriptomes while preserving tissue architecture.
09:32
Development of New Methods for Quantifying Fish Density Using Underwater Stereo-video Tools
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Cited by 9 •
2017
We describe a new method for counting fishes, and estimating relative abundance (MaxN) and fish density using rotating stereo-video camera systems. We also demonstrate how to use distance from camera (Z distance) to estimate species-specific detectability.
14:14
Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics
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Cited by 9 •
2017
This protocol uses multi-view stereo to generate three-dimensional (3D) models out of uncalibrated sequences of photographs, making it affordable and adjustable to a surgical setting. Strain maps between the 3D models are quantified with spline-based isogeometric kinematics, which facilitate representation of smooth surfaces over coarse meshes sharing the same parameterization.
05:54
Stereo-Electro-Encephalo-Graphy (SEEG) With Robotic Assistance in the Presurgical Evaluation of Medical Refractory Epilepsy: A Technical Note
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Cited by 20 •
2016
Stereo-electroencephalography (SEEG) aids in localization of epileptogenic zones, however, remains relatively underutilized in the United States. The goal of this abstract is to provide a brief introduction to the technique of SEEG and further a detailed technique of using robotic assistance in the placement of SEEG electrodes.
04:57
Proper Care and Cleaning of the Microscope
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Cited by 2 •
2008
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
FAQs
The instrument directs light through two separate optical paths, producing slightly different views of the same specimen. Each eye receives one of these perspectives, and the visual system combines them into a three-dimensional impression. This depth information helps users judge surface contours and spatial relationships while examining intact biological samples or carrying out precise manipulation.
Reflected and transmitted illumination support different ways of viewing a specimen. Reflected light helps reveal surface features on objects such as insects, plants, and tissues, while transmitted light illuminates specimens from below. Having both options broadens the types of biological samples that can be observed and supports examination of samples that may be large or opaque.
A stereo microscope is suited to larger, intact, or opaque samples that may not be practical for conventional compound microscopy. Its lower magnification, broad field of view, and substantial working distance favor surface inspection and hands-on manipulation. Compound microscopy is not described here in detail, but the contrast lies in the stereo instrument’s suitability for observing and handling unsectioned specimens.
The relatively low magnification provides a broad field of view rather than concentrating on very small details. This makes it easier to survey intact specimens, locate structures, and maintain space for manipulation. In biology, that balance is useful when examining insects, plants, tissues, or other samples whose overall form and surface features matter alongside individual structures.
Biologists and students can use the instrument during dissections, sorting, and specimen manipulation. Its substantial working distance leaves room to perform precise procedures while viewing the sample, and its three-dimensional image supports spatial judgment. Because specimens can remain intact and need not be sectioned, the method is practical for handling and examining biological material directly.
Insects, plants, tissues, and other intact samples are suitable when the goal is to inspect overall form or surface features. The broad field of view accommodates relatively large specimens, while the optical arrangement provides depth perception. This combination supports both observational work and practical tasks such as sorting, dissection, and manipulation in biology.