Stromal Invasion
Stromal Invasion - Related Videos
05:48
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
0 Views •
Cited by 7 •
2014
Tissue engineered fibroblast-derived native matrix is an emerging tool to generate a stromal substrate which supports epithelial cell proliferation and differentiation. Here a protocol applying this methodology to assess the impact of different stromal cell types on tumor cell biology is presented.
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 86 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
09:18
Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion
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2025
There is a critical need for 3D cancer models that capture heterocellular crosstalk to study cancer metastasis. Our study presents the generation of heteromulticellular stromal-epithelial in a scaffold and scaffold-free environment that can be used to study invasion and cellular spatial distributions.
07:06
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Cited by 171 •
2017
Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
08:08
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
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Cited by 16 •
2014
This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.
FAQs
Progression requires more than cell movement alone. Abnormal cells must breach the basement membrane, alter or degrade extracellular matrix, and migrate through connective tissue. These changes create a physical route from the epithelial compartment into the surrounding stroma. The sequence is important because it connects altered tissue architecture with increasingly invasive tumor behavior.
Cancer-associated fibroblasts and inflammatory cells can support invasion by participating in the altered tumor microenvironment. Their presence reflects interactions between abnormal cells and surrounding host tissue, while signaling between these populations may influence matrix remodeling and cell motility. Consequently, invasion is not solely a property of tumor cells; it can depend on cooperation within the surrounding stroma.
The basement membrane marks an important boundary between an epithelial compartment and underlying connective tissue. Its breach provides evidence that abnormal cells have crossed beyond their original compartment, especially when accompanied by extracellular-matrix remodeling and movement through stromal tissue. This distinction helps separate localized disease from invasive cancer during biological and pathological assessment.
A localized epithelial abnormality remains confined to its original epithelial compartment, whereas stromal invasion is associated with penetration beyond that boundary into connective tissue. The difference therefore depends on tissue compartment, basement-membrane integrity, and evidence of stromal migration. In pathology, this distinction contributes to tumor assessment because invasive behavior can affect grading and prognosis.
Assessment focuses on the relationship between abnormal epithelial cells and the surrounding tissue. Investigators look for penetration across the basement membrane, changes or degradation in extracellular matrix, and movement of cells through stromal tissue. They may also consider the surrounding cellular environment, including cancer-associated fibroblasts and inflammatory cells, to interpret tumor-stroma interactions.
Identifying Stromal Invasion helps distinguish localized disease from invasive cancer and supports evaluation of tumor progression. In pathology, the finding can contribute to grading and prognosis. In research, it provides a framework for studying how malignant cells interact with connective tissue, including processes linked to the tumor microenvironment and potential progression toward metastasis.
Research commonly examines cell motility, extracellular-matrix remodeling, and signaling between tumor cells and host stromal cells. These processes are studied together because invasion depends on both movement through tissue and changes to the surrounding environment. The resulting knowledge can support investigations of metastasis and therapies directed at matrix remodeling, motility, or tumor-stroma interactions.