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Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
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Bioengineering
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JoVE Journal Bioengineering
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

DOI:
10.3791/51321-v

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05:48 min

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March 18, 2014

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,
1Division of Cancer Research, Ninewells Hospital and Medical School,University of Dundee, 2Institute of Medical Biology,A*Star, Singapore

Chapters

  • 00:05Title
  • 01:20In Vitro Construction of Fibroblast-derived Native Matrix
  • 03:01Invasion Assay with Tumor Squamous Cell Carcinoma (SCC) Keratinocytes
  • 04:47Results: Representative cSCC Invasion into the Native Matrix
  • 05:24Conclusion

Summary

Automatic Translation

Automatic Translation

Tissue engineered fibroblast-derived native matrix is an emerging tool to generate a stromal substrate which supports epithelial cell proliferation and differentiation. Here a protocol applying this methodology to assess the impact of different stromal cell types on tumor cell biology is presented.

Tags

Tumor Stromal Microenvironment3D Organotypic CulturesExtracellular MatrixType I CollagenEngelbreth-Holm-Swarm Mouse SarcomaDermal FibroblastsRecessive Dystrophic Epidermolysis BullosaNative MatrixEpithelial-mesenchymal Cell InteractionsType VII CollagenTumor Cell Invasion

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