Vortexing Method

The vortexing method is a laboratory mixing technique that rapidly combines liquids or resuspends biological samples, helping produce uniform preparations for reliable analysis. A tube or vessel is placed on a vortex mixer, whose rotating platform transfers orbital motion to the liquid and generates a vortex; the resulting fluid movement and shear distribute cells, particles, and dissolved reagents throughout the sample. In biology, vortexing supports cell suspension, reagent preparation, nucleic acid workflows, and recovery of material from pellets or tube walls. Mixing speed and duration must be controlled because excessive shear can damage fragile cells or promote foaming, affecting downstream measurements and experimental reproducibility.

Vortexing Method - Related Videos

Research

JoVE Journal - Environment
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Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

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2025

This study presents the methodology for the generation of six different types of plasma discharges within a Hyperbolic Vortex Plasma Reactor for the degradation of micropollutants in water, including pharmaceuticals and per- and polyfluoroalkyl substances (PFAS).

Research

JoVE Journal - Bioengineering

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Cited by 2 •

2014

A microfluidic vortex assisted electroporation platform was developed for sequential delivery of multiple molecules into identical cell populations with precise and independent dosage control. The system’s size based target cell purification step preceding electroporation aided to enhance molecular delivery efficiency and processed cell viability.

Education

JoVE Science Education - Engineering

Flow Visualization in a Water Tunnel: Observing the Leading-edge Vortex Over a Delta Wing

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2023

Source: Jose Roberto Moreto, Gustaaf Jacobs and Xiaofeng Liu, Department of Aerospace Engineering, San Diego State University, San Diego, California The delta wing, shown in Figure 1D, is a popular design in high-speed airplanes due to its superb performance in transonic and supersonic flight regimes. This type of wing has a small aspect ratio and high sweep angle, which reduces drag at high subsonic, transonic and supersonic flight regimes. The aspect ratio is defined as the wing span divided...

Scanning SQUID Study of Vortex Manipulation by Local Contact

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Cited by 1 •

2017

We present a protocol for manipulation of individual vortices in thin superconducting films, using local mechanical contact. The method does not include applying current, magnetic field or additional fabrication steps.

Noninvasive Determination of Vortex Formation Time Using Transesophageal Echocardiography During Cardiac Surgery

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2018

We describe a protocol to measure vortex formation time, an index of left ventricular filling efficiency, using standard transesophageal echocardiography techniques in patients undergoing cardiac surgery. We apply this technique to analyze vortex formation time in several groups of patients with differing cardiac pathologies.

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