Gfp-labeled Cancer Cells
Gfp-labeled Cancer Cells - Related Videos
04:55
CRC Organoid Cell Labeling: A Method to Generate GFP Lentivirus-transduced Colorectal Cancer Organoid Cells
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2023
This video describes the technique of generating CRC organoids transduced by GFP lentiviral particles for enabling fluorescent imaging. These GFP labeled organoids, when placed in animal models, help to study tumor invasion and metastasis.
07:25
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
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Cited by 5 •
2013
A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.
05:08
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
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2023
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
07:59
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2 •
2016
We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.
09:57
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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Cited by 11 •
2015
Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.
FAQs
The introduced GFP gene directs production of green fluorescent protein inside the engineered cells. When researchers illuminate the sample at an appropriate excitation wavelength, the protein emits green light that distinguishes labeled cells from surrounding material. This optical signal allows cancer-cell location and behavior to be monitored directly in experimental systems rather than revealed through a separate staining step.
Because the marker remains associated with the engineered cancer cells, researchers can observe changes in their distribution and activity during an experiment. Sequential imaging can reveal patterns related to proliferation, migration, invasion, metastasis, or treatment response. This time-based view adds information about tumor-cell behavior that a single endpoint measurement may not provide.
GFP labeling produces an internally expressed fluorescent marker, whereas staining requires researchers to apply a detection reagent to a sample. The GFP signal can identify cells during imaging without staining each sample, supporting observation within ongoing experimental systems. This distinction is especially relevant when investigators need to track tumor cells across changing conditions or treatment periods.
The fluorescent signal supports quantitative tracking of several tumor-related behaviors, including cell proliferation, movement, invasion, metastatic spread, and response to treatment. Researchers can compare the distribution or behavior of labeled cells across experimental conditions and use those measurements to evaluate how a therapy or model influences cancer progression.
Researchers first work with cancer cells carrying the introduced GFP gene, then place them in an experimental system such as a culture model or an animal model. They illuminate the system at an appropriate wavelength, detect the emitted green fluorescence, and follow the labeled cells during the study. The resulting images support observation and quantitative analysis.
They are useful when investigators need to visualize tumor-cell behavior in culture or animal models. Applications include examining how cancer cells proliferate, migrate, invade surrounding settings, form metastatic patterns, or respond to treatment. By making these processes visible, the labeled cells help connect experimental conditions with measurable changes in cancer-cell distribution and behavior.