Macrophage Migration
Macrophage Migration - Related Videos
02:38
Live Imaging of Phagosome Migration in HIV-1-Infected Human Macrophages
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2026
Source: Lê-Bury, G., et al. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (2016)This video demonstrates live confocal imaging of HIV-1-infected human macrophages to visualize and quantify phagosome migration using GFP fluorescence and bright field tracking, providing insights into how HIV alters intracellular trafficking during Fc receptor–mediated phagocytosis.
07:32
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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Cited by 4 •
2016
We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Cited by 326 •
2013
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
2016
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
FAQs
Chemokines and other chemoattractants bind receptors on macrophages and initiate intracellular signaling. These signals reorganize the actin cytoskeleton, which supports changes in cell shape and movement, while adhesion to the extracellular matrix provides traction. Together, receptor signaling, cytoskeletal remodeling, and matrix adhesion help macrophages move toward regions with higher chemoattractant concentrations.
Actin cytoskeletal remodeling enables macrophages to generate the structural changes needed for movement, whereas adhesion to the extracellular matrix helps cells maintain contact with surrounding tissue as they advance. Neither process acts alone: signaling from chemoattractant receptors coordinates both, linking environmental detection with the physical steps required for directed movement.
Macrophage entry and distribution within tumors can affect several features of cancer biology. Once positioned in the tumor microenvironment, macrophages may influence angiogenesis, immune suppression, invasion, and metastasis. Studying their migration therefore connects the cellular response to environmental signals with broader changes in tumor progression and the organization of immune cells around cancer tissue.
Migration measurements can help determine how macrophages respond to environmental signals and how efficiently they enter or distribute within tumor tissue. These observations provide a way to examine relationships between macrophage recruitment and tumor progression. They can also help researchers evaluate whether an intervention changes macrophage positioning or the functions associated with that positioning.
Researchers can manipulate the signals or cellular processes that control macrophage recruitment and movement, then assess the resulting changes in tumor-associated macrophage entry or distribution. Such experiments support investigation of how migration relates to angiogenesis, immune suppression, invasion, or metastasis, and they provide a basis for evaluating approaches intended to alter macrophage recruitment or function.
Macrophage migration becomes especially relevant when a therapy aims to change which macrophages reach a tumor, where they accumulate, or how they function after recruitment. Measuring migration before and after manipulation can connect treatment effects with tumor-associated macrophage behavior. This helps frame strategies that target recruitment or function as part of studies on tumor progression.