Ovarian Tissue Explants
Ovarian Tissue Explants - Related Videos
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
03:18
Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants
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2023
In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.
11:51
Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
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Cited by 3 •
2019
This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.
10:57
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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2017
Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
FAQs
Maintaining native three-dimensional architecture allows epithelial, stromal, vascular, and immune cells to remain positioned within their tissue context. That organization supports interactions and signaling patterns that may be altered when cells are studied in isolation. In cancer research, this structure helps investigators examine tissue remodeling and treatment responses while retaining more of the original ovarian microenvironment.
These cell populations contribute distinct components to the explant’s local signaling environment. Their continued presence enables researchers to study communication between ovarian tissue compartments rather than focusing only on malignant or epithelial cells. This broader cellular context is particularly relevant for investigating how the tumor microenvironment may influence tumor initiation, tissue remodeling, and responses to experimental treatments.
Ovarian tissue explants provide an intermediate research model that preserves native tissue interactions more effectively than isolated cell systems while remaining outside the body. They can complement, rather than replace, cell-line and animal studies by offering a way to monitor treatment responses within partially intact ovarian architecture. This helps connect simplified laboratory findings with tissue-level behavior.
Researchers place pieces of ovarian tissue in a controlled culture medium containing appropriate nutrients and maintain suitable environmental conditions. The explants are then exposed to the experimental treatment or comparison condition, while investigators monitor tissue responses over the course of the study. This workflow preserves the tissue context sufficiently to evaluate changes in signaling, remodeling, or treatment response.
The model supports studies of tumor initiation, remodeling within ovarian tissue, hormone-related effects, and communication across the tumor microenvironment. Because multiple tissue compartments remain present, investigators can examine how experimental conditions affect relationships among cells rather than measuring only isolated-cell behavior. These applications make explants useful for exploring mechanisms that shape ovarian cancer development and progression.
Researchers can apply experimental treatments to cultured explants and monitor responses in a tissue setting that retains several native cellular interactions. This approach can help evaluate treatment effects before or alongside studies using cell lines and animal models. The same tissue-level context can support biomarker investigations by linking observed responses with changes occurring within the ovarian microenvironment.