Patient-derived Culture
Patient-derived Culture - Related Videos
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
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Cited by 22 •
2013
This article describes the preparation of freshly obtained melanoma tissue into primary cell cultures, and how to remove contaminations of erythrocytes and fibroblasts from the tumor cells. Finally, we describe how CD133+ putative melanoma stem cells are sorted from the CD133- bulk using Magnetic Activated Cell Sorting (MACS).
08:29
Establishment and Culture of Patient-Derived Breast Organoids
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Cited by 7 •
2023
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
09:24
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
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Cited by 20 •
2016
Patient-derived xenografts of glioblastoma multiforme can be miniaturized into living microtumors using 3D human biogel culture system. This in vivo-like 3D tumor assay is suitable for drug response testing and molecular profiling, including kinomic analysis.
03:21
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
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2023
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
07:55
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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Cited by 16 •
2018
The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management.
FAQs
Growth signals and nutrients determine whether collected tumor cells remain viable and proliferate after removal from the patient. In the culture environment, these inputs support cellular maintenance and growth under controlled laboratory conditions. Their role is not merely to keep samples alive; they help create a workable model in which researchers can observe tumor growth and cellular behavior ex vivo.
Patient-derived Culture can retain some features of the original sample, allowing experiments to reflect aspects of patient-specific tumor biology. This preservation supports analysis of cellular behavior, growth, and treatment response in a laboratory setting. Because the model maintains only some original characteristics, researchers use it as an informative experimental representation rather than a complete reproduction of the tumor.
Researchers can expose the cultured tumor material to treatments and examine how the cells respond. Differences in growth or cellular behavior can provide evidence of therapeutic sensitivity, while continued growth under treatment can support studies of resistance mechanisms. These observations help connect patient-specific experimental findings with investigations of treatment response in cancer research.
The process begins with collecting tumor material from an individual and processing the tissue or cells for laboratory use. The prepared material is then placed in a suitable culture environment and supplied with nutrients and growth signals. Under controlled conditions, viable cells may proliferate, creating a system for subsequent observation of growth, behavior, or treatment response.
Maintaining these cultures requires controlled laboratory conditions, a suitable environment, and support from nutrients and growth signals. Together, these factors help preserve cell viability and permit proliferation when the collected material can grow ex vivo. Researchers rely on this controlled setting to make observations about tumor behavior and therapeutic responses under consistent experimental conditions.
This approach supports disease modeling, drug screening, biomarker evaluation, and studies of treatment response. Researchers can use the cultures to examine tumor growth and cellular behavior while testing how patient-derived material responds to therapeutic conditions. The resulting information may help connect experimental cancer research with investigations of personalized care and patient-specific treatment decisions.