Phoenix Cells
Phoenix Cells - Related Videos
11:26
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
0 Views •
Cited by 19 •
2015
This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.
13:23
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
0 Views •
Cited by 13 •
2012
We present a protocol for efficient reprogramming of human somatic cells into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) and identification of correctly reprogrammed hiPSC by live staining with Tra-1-81 antibody.
09:19
Recombinant Retroviral Production and Infection of B Cells
0 Views •
Cited by 2 •
2011
An efficient system of structure and function analysis of a gene in an ex vivo culture of splenic B-lymphocytes is described. This method takes advantage of recombinant retroviral production in a helper free, ecotrophic packaging cell line. Stable, heritable expression of a gene of interest within primary lymphocytes is achieved leading to generation of surface antibodies on B cells undergoing class switch recombination.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
0 Views •
Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
09:16
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
0 Views •
Cited by 22 •
2015
The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.
FAQs
The system separates cargo from packaging support: the introduced retroviral vector carries the gene of interest, whereas Phoenix Cells provide gag, pol, and envelope functions needed to assemble particles. This division allows the vector’s cargo to be packaged and delivered while supporting functions remain supplied by the cells, making the system suitable for controlled gene transfer into cancer models.
Replication incompetence means the particles are intended to deliver the vector to target cells rather than establish a self-propagating viral process. In cancer research, this focuses experiments on the introduced gene and its effects, including oncogene function, tumor-suppressor activity, signaling pathways, and drug response, while keeping the system centered on gene delivery rather than viral propagation.
These functions provide the viral support required for particle production. Gag and pol are supplied among the structural and enzymatic components, while envelope functions contribute to the assembled particles’ ability to deliver vector cargo to target cells. Together, they allow the vector carrying the gene of interest to be converted into usable retroviral particles.
Researchers first introduce the retroviral vector into Phoenix Cells. The cells then supply the required viral structural and enzymatic functions, assemble particles, and release them into the culture medium. That particle-containing medium is subsequently used to transduce target cells, creating a direct workflow from vector introduction to gene delivery in experimental models.
Stable gene expression allows investigators to examine the consequences of introducing a selected gene into cancer cell models. This supports focused studies of oncogene function, tumor-suppressor activity, and signaling pathways, as well as assessments of drug response. The resulting models help connect a gene or pathway perturbation with cancer-related experimental outcomes.
Phoenix Cells support experiments that alter gene activity in cancer cell models and then evaluate the resulting biological effects. Researchers can examine how oncogenes contribute to cancer-related behavior, how tumor suppressors influence cells, how signaling pathways function, and how these changes affect responses to drugs. The same delivery approach can therefore address several complementary research questions.
The ability to deliver vector-encoded genes into target cells makes Phoenix Cells useful for genome-scale functional screens. Such studies can evaluate the effects of many gene perturbations across cancer models, helping identify genes or pathways associated with cellular behavior, tumor-suppressor activity, oncogenic function, or drug response. Stable expression supports analysis of the resulting phenotypes.