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JoVE Journal
Immunology and Infection

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Recombinant Retroviral Production and Infection of B Cells
 

Recombinant Retroviral Production and Infection of B Cells

Article doi: 10.3791/2371
February 18th, 2011 Usage Statistics

Summary February 18th, 2011

An efficient system of structure and function analysis of a gene in an ex vivo culture of splenic B-lymphocytes is described. This method takes advantage of recombinant retroviral production in a helper free, ecotrophic packaging cell line. Stable, heritable expression of a gene of interest within primary lymphocytes is achieved leading to generation of surface antibodies on B cells undergoing class switch recombination.

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