Serial Fraction Collection
Serial Fraction Collection - Related Videos
08:08
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
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Cited by 272 •
2008
Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.
11:06
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
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Cited by 16 •
2017
Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.
06:19
Fixed Target Serial Data Collection at Diamond Light Source
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Cited by 22 •
2021
We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
01:03
Serial Position Effect
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2025
The serial position effect is a cognitive phenomenon where individuals are more likely to recall the first and last items in a list compared to those in the middle. This effect is divided into the primacy effect and the recency effect. The primacy effect is observed when the initial items in a list are remembered better. This occurs because these items are rehearsed more frequently or receive more elaborative processing, allowing them to be encoded into long-term memory more effectively. For...
FAQs
The collector can change the receiving well or tube at preset time intervals, producing a regular sequence of eluate portions. Alternatively, a detector can trigger switching as components emerge from the separation system. These control modes let researchers choose between uniform time-based sampling and collection linked to detected elution behavior, depending on how they want to preserve differences among emerging components.
Components that leave the separation system at different times enter different labeled fractions, preserving their retention differences. Because each portion remains associated with its collection position, researchers can compare concentration, protein or metabolite content, and biological effects across the sequence. This positional information helps connect an observed cancer-relevant signal with the fraction in which it appears.
A biological effect can be tested fraction by fraction rather than only in the unfractionated sample. If one portion shows cytotoxicity or another cancer-relevant activity, its label identifies the corresponding chemical or molecular fraction for further characterization. The approach connects screening results with separation behavior, supporting the search for candidate therapeutic compounds.
Successive eluate volumes are directed into separately labeled wells or tubes as they emerge. The individual portions can then be screened for cytotoxicity, protein content, metabolite content, bioactivity, or other cancer-relevant signals. Keeping fractions separate allows each measured result to be related to its position in the sequence and to the material that emerged at that time.
This approach is useful when a sample must be examined across its separated components rather than as a single mixture. The workflow supports purification, biomarker characterization, and activity-guided identification of candidate therapeutic compounds. By preserving separate fractions, it enables researchers to investigate whether a cancer-relevant signal is associated with a particular portion of the separation.
Fraction-by-fraction screening can reveal where cytotoxicity, proteins, metabolites, bioactivity, or other cancer-relevant signals occur within the collected sequence. These results associate a measured property with a separated fraction rather than describing the sample only globally. That association can guide biomarker characterization or help identify molecular material linked to a potential therapeutic effect.