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Fixed Target Serial Data Collection at Diamond Light Source
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Fixed Target Serial Data Collection at Diamond Light Source

Fixed Target Serial Data Collection at Diamond Light Source

DOI:
10.3791/62200-v

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06:19 min

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February 26, 2021

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, , , , , , , , ,
1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences,University of Essex, 3X-ray Science Division,Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences,University of Southampton

Chapters

  • 00:04Introduction
  • 00:59Preparing and Loading a Chip
  • 02:53Aligning the Chip
  • 04:18Results: Data Indexing and Integration and Visualization of Unit Cell Parameters
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.

Tags

Keywords: Serial Data CollectionFixed TargetSerial Synchrotron CrystallographySSXLow DoseRoom TemperatureProtein DynamicsSilicon ChipPolyester FoilChip HolderChip LoadingMicrocrystal SlurryVacuumAlignmentKinematic MountXYZ StageOn-axis Viewing

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