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A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
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A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

DOI:
10.3791/960-v

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08:08 min

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November 10, 2008

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,
1Department of Pathology,Columbia University

Chapters

  • 00:00Title
  • 01:02Introduction
  • 02:10Making the Glass Capillary
  • 02:52Anesthetizing the Mice
  • 03:19Preparing the Mouse for CSF Sampling
  • 04:42Collecting CSF Using the Cisterna Magna Puncture Technique
  • 06:03After CSF Sampling
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.

Tags

Cerebrospinal FluidCSFCisterna MagnaMouseAlzheimer’s DiseaseADNeurodegenerative DiseaseAmyloid PeptideTau ProteinBiochemical ChangesTransgenic Mouse ModelsPuncture TechniqueSamplingAβ42Tau LevelsDisease ProgressionELISAMetabolism

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