Tumor Sample Processing
Tumor Sample Processing - Related Videos
07:32
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Cited by 36 •
2012
Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.
03:39
Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)
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2023
This protocol describes the preparation of formalin-fixed paraffin-embedded tumor samples which are used to preserve tissue and cytology samples from non-small cell lung cancer...
05:07
Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
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Cited by 20 •
2018
The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types.
04:03
Protein Extraction and Proteolysis: A Method to Obtain Proteins from Prostate Tumor Tissue Samples and their Enzymatic Digestion into Peptides
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2023
This video describes the technique of protein extraction and digestion to obtain peptides from prostate tumor tissue. These peptides on further analysis can help identify novel targets for oncotherapy.
11:20
In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
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Cited by 1 •
2017
This article describes a setup and method for the in situ visualization of oil samples under a variety of temperature and pressure conditions that aim to emulate refining and upgrading processes. It is primarily used for studying isotropic and anisotropic media involved in the fouling behavior of petroleum feeds.
FAQs
The handling condition during transport can determine whether a specimen remains suitable for later analysis. Tumor sample processing must therefore preserve the cellular, molecular, and structural features required by the planned assay. Controlled transport and preparation support more reliable histopathology, molecular profiling, and cell-based studies, while inconsistent handling can weaken comparisons between specimens.
Processing format should match the information a study needs. Fixation supports preservation of tissue architecture for histopathology and immunostaining, freezing preserves material for molecular analyses, and mechanical or enzymatic dissociation produces single-cell suspensions for flow cytometry or primary cell culture. Choosing among these routes determines which structural, cellular, or molecular measurements remain accessible.
Consistent handling is especially important because tumors can contain diverse cellular and molecular features. Dividing material into appropriately prepared portions allows different assays to examine complementary aspects of the same specimen. Standardized processing improves comparability across tumors and helps researchers interpret biomarker patterns, disease mechanisms, and treatment responses without confusing handling differences with biological variation.
A typical workflow begins with controlled transport, followed by division and selection of preparation conditions based on the intended analysis. Material may then be fixed, frozen, or dissociated; filtration and centrifugation can remove debris and enrich usable material when suspensions are prepared. The resulting fractions are directed to structural, molecular, or cell-based assays.
When dissociation is used, filtration and centrifugation help refine the resulting suspension. Filtration removes debris, while centrifugation supports enrichment of usable material. These steps are relevant when researchers need cleaner single-cell preparations for flow cytometry or primary cell culture. Their value lies in increasing the fraction of material available for downstream analysis.
In cancer research, processed tumor material can support several complementary readouts: histopathology examines tissue structure, immunostaining evaluates features in tissue, and genomic or transcriptomic profiling characterizes molecular information. Flow cytometry and primary cell culture extend analysis to cell populations and cultured material. Suitable preparations help characterize heterogeneity, identify biomarkers, and evaluate treatment responses.