Animal Tissue Analysis
Animal Tissue Analysis - Related Videos
08:53
The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering
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Cited by 38 •
2016
We describe a microsurgical approach for the generation of an arteriovenous (AV) loop as a model for analyzing vascularization in vivo in an isolated and well-characterized environment. This model is not only useful for the investigation of angiogenesis, but is also optimally suited for engineering axially vascularized and transplantable tissues.
11:00
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
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Cited by 8 •
2010
Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.
06:23
Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo
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Cited by 1 •
2018
Here we present a protocol to experimentally assess plasma coagulation in liver tissue in vivo. In a porcine model, microcirculation is examined by laser Doppler, coagulation depth is measured histologically, temperature at coagulation site by infrared thermometer and thermographic camera, and duct sealing effect is documented by burst pressure experiments.
12:53
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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Cited by 1 •
2018
This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA analysis.
14:59
Sampling and Analysis of Animal Scent Signals
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Cited by 7 •
2021
We have developed an effective methodology for sampling and analysis of odor signals in order to understand how they may be used in animal communication. Particularly, we use headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry to analyze the volatile components of animal odors and scent-markings.
FAQs
Chemical measurements identify and quantify constituents such as proteins, lipids, metabolites, drugs, and elemental components, whereas structural examination shows how those findings relate to tissue organization. Combining both perspectives can connect molecular changes with physiological, disease-related, or treatment-related alterations. This relationship helps researchers interpret whether a measured chemical difference corresponds to a particular tissue context.
Tissue contains a complex biological matrix that can affect how analytes are released, separated, and measured. Homogenization helps produce a more uniform sample, while extraction separates target substances from other tissue components before instrumental analysis. Managing the matrix is therefore important for obtaining measurements that can be compared across tissues and linked to biological conclusions.
These methods provide complementary chemical measurements. Spectrophotometry measures analytes through their interaction with light, chromatography separates components within a sample, and mass spectrometry measures analytes according to their mass-related characteristics. Selecting among them depends on the substances being examined and the type of measurement required. Microscopy can add structural context to the resulting chemical data.
A chemistry-based workflow begins with reliable tissue sampling and preservation, followed by homogenization to prepare the material for analysis. Researchers then extract and separate analytes from the biological matrix before measuring them with an appropriate analytical method. Maintaining consistent preparation across samples supports meaningful comparisons and reduces the risk that handling differences will obscure biological or treatment-related changes.
Calibration connects an instrument response with the amount of analyte being measured, while controls provide reference conditions for evaluating the result. Together with consistent sampling and extraction, they help distinguish genuine differences among tissues from variation introduced by the analytical workflow. These safeguards are particularly important when measurements are used to interpret physiology, disease, toxicology, or treatment effects.
The approach supports investigations of metabolism, toxicology, pharmacology, and disease mechanisms by measuring chemical constituents within tissue samples. Researchers may examine proteins, lipids, metabolites, drugs, or elemental constituents, then relate the measurements to tissue structure through microscopy when appropriate. The resulting data can help evaluate physiological changes, disease-associated alterations, or responses to treatment.