Aqueous Phase

An aqueous phase is the portion of a chemical system in which water serves as the solvent, providing a medium for dissolved ions and molecules. Polar water molecules stabilize solutes through hydration and hydrogen bonding, while dissociation, diffusion, acid–base equilibria, and ion interactions determine how substances behave in solution. In chemistry, aqueous phases support precipitation, complex formation, titrations, redox reactions, and biological processes, and they commonly participate in liquid–liquid extraction alongside an organic phase. Understanding composition, concentration, pH, and ionic strength helps researchers predict solubility, reaction pathways, phase separation, and the recovery or purification of chemical compounds.

Aqueous Phase - Related Videos

Research

JoVE Journal - Bioengineering

Cell Co-culture Patterning Using Aqueous Two-phase Systems

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Cited by 32 •

2013

Aqueous two-phase systems were used to simultaneously pattern multiple populations of cells. This fast and easy method for cell patterning takes advantage of the phase separation of aqueous solutions of dextran and polyethylene glycol and the interfacial tension that exists between the two polymer solutions.

Robotic Production of Cancer Cell Spheroids with an Aqueous Two-phase System for Drug Testing

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Cited by 37 •

2015

A protocol for robotic printing of cancer cell spheroids in a high throughput 96-well plate format using an aqueous two-phase system is presented.

Education

JoVE Core - Chemistry

Chemical Reactions in Aqueous Solutions

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2020

Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction. Water is a good solvent that can dissolve many substances. For this reason, many chemical reactions take place in water. Such reactions are called aqueous reactions. The three most common types of aqueous reactions are precipitation, acid-base, and...

Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures

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Cited by 4 •

2017

A protocol for determining the vitreous phase densities of micro- to pico-liter size drops of aqueous mixtures at cryogenic temperatures is described.

Aqueous Solutions and Heats of Hydration

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2020

Water and other polar molecules are attracted to ions. The electrostatic attraction between an ion and a molecule with a dipole is called an ion-dipole attraction. These attractions play an important role in the dissolution of ionic compounds in water. When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...

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