Colorectal Carcinoma Xenograft
Colorectal Carcinoma Xenograft - Related Videos
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
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Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
08:17
The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma
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Cited by 62 •
2015
The chick chorioallantoic membrane (CAM) is immunodeficient and highly vascularized, making it a natural in vivo model of tumor growth and angiogenesis. In this protocol, we describe a reliable method of growing three-dimensional, vascularized hepatocellular carcinoma (HCC) tumors using the CAM assay.
03:19
Generating PDOX Mouse Model of Colorectal Cancer: Studying Progression of Colorectal Cancer in a Murine Model
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2023
This video describes the protocol for generating a patient-derived orthotopic xenograft colorectal cancer mouse model to study the progression of colorectal cancer cells in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. This murine model helps in understanding primary tumor growth and metastases pattern.
07:13
Comparison of Predictive Performance of Three Lymph Node Staging Systems in Colorectal Signet Ring Cell Carcinoma Based on Machine Learning Model
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Cited by 1 •
2025
This study evaluates prognostic systems for colorectal signet-ring cell carcinoma patients using machine learning models and competing risk analyses. It identifies log odds of positive lymph nodes as a superior predictor compared to pN staging, demonstrating strong predictive performance and aiding clinical decision-making through robust survival prediction tools.
03:32
Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma
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2023
This video describes the procedure to generate an experimental xenograft murine model, which can be used for studying urothelial cell carcinoma progression.
FAQs
Reduced immune activity limits rejection of the implanted human colorectal cancer cells or tumor tissue. This permits the graft to survive and form a tumor within the animal, creating a living setting in which researchers can examine tumor growth and responses to experimental treatment rather than measuring activity only in isolated cells.
The resulting tumor can retain key features of colorectal carcinoma while growing in a living system. That preservation gives researchers a basis for relating treatment exposure to tumor behavior, including progression, delayed growth, or regression, and helps connect observations from the model with the biology of the implanted cancer.
Common outcomes identified for this model include tumor growth, drug exposure, therapeutic efficacy, and toxicity. Together, these measurements show not only whether an experimental treatment affects the tumor, but also whether the compound reaches a relevant biological setting and produces undesirable effects that should be considered before clinical studies.
They help connect a compound's chemical structure with biological activity by supplying measurable tumor responses in vivo. Researchers can compare experimental compounds through outcomes such as tumor regression or delayed progression, while also examining exposure, efficacy, and toxicity. This makes the model useful for prioritizing chemical candidates before clinical evaluation.
The workflow begins by implanting human colorectal cancer cells or tumor tissue into an immunodeficient animal. Researchers then allow the graft to form a tumor and evaluate its growth, drug exposure, response to experimental treatment, and toxicity. These observations provide the in vivo results used to assess a candidate treatment before clinical studies.
Researchers use colorectal carcinoma xenografts when they need evidence from a living system before clinical studies. The model is particularly relevant when evaluating how an experimental treatment affects tumor growth and whether it produces regression or delayed progression, while also recording drug exposure, therapeutic efficacy, and toxicity in the same research context.