Intestine Liver Chip
Intestine Liver Chip - Related Videos
10:51
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
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Cited by 68 •
2016
We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
05:53
Use of a Hanging-weight System for Liver Ischemia in Mice
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Cited by 4 •
2012
We established a novel murine model of a hanging weight system for portal triad occlusion. This technique may be useful for future investigations of ischemia in murine hepatic models.
08:59
An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
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Cited by 3 •
2020
We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.
FAQs
Controlled microfluidic flow creates a connected exchange between intestinal and hepatic compartments. Nutrients, metabolites, drugs, and other compounds can move from one tissue model to the other instead of being evaluated in isolation. This arrangement makes it possible to examine how an intestinal exposure is modified by the liver and how resulting chemicals may influence downstream tissue responses.
Tissue-specific barriers help preserve functions that isolated cell cultures may not represent adequately. Maintaining those barriers while compounds move under controlled flow supports more realistic assessment of intestinal absorption, hepatic metabolism, toxicity, and metabolite formation. For chemistry and pharmaceutical studies, this matters because the measured response reflects communication between linked tissues rather than a single compartment alone.
Compound behavior can be examined by following its movement between connected compartments while controlled flow and tissue-specific functions are maintained. This approach links exposure with intestinal absorption, hepatic metabolism, toxicity, and metabolite formation. It also helps distinguish effects associated with gut-liver interaction from observations made in an isolated cell culture.
Researchers culture living intestinal and hepatic cells in connected microfluidic compartments, establish controlled flow, and expose the system to nutrients, metabolites, drugs, or other compounds. They then assess movement between tissues and measure responses such as absorption, hepatic metabolism, toxicity, or metabolite formation. The workflow preserves tissue-specific barriers and functions during testing.
It can reveal how a compound crosses the intestinal model, how the liver transforms it, whether toxicity emerges, and which metabolites form. Because the tissues are linked, these measurements capture chemical exchange across the gut-liver axis rather than only a response from one isolated cell type. The resulting information can support interpretation of compound behavior in pharmaceutical research.
This platform is particularly relevant when researchers need to evaluate compounds in the context of intestinal and hepatic interaction. Chemistry and pharmaceutical teams can apply it during compound optimization, when assessing absorption, metabolism, toxicity, or metabolite formation, and when studying how chemical exposures alter organ communication. Its use can also improve human-response predictions while reducing reliance on animal studies.