On-resin Peptide Functionalization
On-resin Peptide Functionalization - Related Videos
14:28
Peptide-based Identification of Functional Motifs and their Binding Partners
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Cited by 2 •
2013
Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.
15:33
Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
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Cited by 32 •
2013
This video will illustrate a rapid, efficient method to methacrylate poly(ethylene glycol), enabling chain polymerizations and hydrogel synthesis. It will demonstrate how to similarly introduce methacrylamide functionalities into peptides, detail common analytical methods to assess functionalization efficiency, provide suggestions for troubleshooting and advanced modifications, and demonstrate typical hydrogel characterization techniques.
11:42
Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
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Cited by 2 •
2012
The efficient solid-phase peptide synthesis of a functionalized bis-peptide trimer utilizing a "safety catch" cleavage procedure from HMBA resin is described.
02:38
Anion Exchange Resin–Based Detection of Viruses from Bioaerosols
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2026
Source: Schaeffer, J. W., Chandler, et al. Detection of Viruses from Bioaerosols Using Anion Exchange Resin. J. Vis. Exp. (2018).This video demonstrates the detection of viruses from bioaerosols using an anion exchange resin. A liquid impinger containing the resin is used to capture viruses from the air. The resin is collected and treated with a virus lysis buffer containing carrier RNA. The lysate is transferred to a microcentrifuge tube for viral RNA isolation, followed by qRT-PCR to amplify...
07:51
Acrylic Resin Molding Based Head Fixation Technique in Rodents
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Cited by 4 •
2016
Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.
FAQs
Protecting groups temporarily mask peptide side chains or other reactive sites while a chosen position remains available for modification. Selective deprotection then exposes the free N-terminus or a targeted side-chain group without broadly unmasking the sequence. This chemoselective control helps direct fluorescent labels, lipid chains, chemical handles, or other modifications to their intended locations.
The polymer support keeps the peptide covalently attached while reagents contact its accessible reactive groups. After a reaction, filtration and washing remove excess reagents and byproducts without requiring the peptide to be isolated from solution. Repeating this reaction and cleanup cycle supports sequential functionalization and limits handling of fragile or partially modified intermediates.
Modification before cleavage allows functional groups to be installed while the peptide remains anchored and purification between individual reaction steps is unnecessary. The resin-bound format also permits selective deprotection and repeated washing during synthesis. Consequently, labels, lipid chains, post-translational modifications, and cyclization precursors can be introduced before the completed peptide is released.
A peptide is first extended on the polymer support through coupling steps, then a selected N-terminal or side-chain protecting group is removed when its reactive site is needed. The desired functionalizing reagent is applied, followed by filtration and washing. These operations can be repeated at appropriate synthesis stages before final cleavage produces the modified peptide.
Supported examples include fluorescent labels for detection, lipid chains for altering peptide properties, chemical handles for later transformations, post-translational modifications for biomolecular studies, and groups that serve as precursors to cyclization. The selected reagent and deprotection sequence determine which peptide site is available, allowing different functional objectives within the same resin-bound synthesis.
The approach is useful when researchers need tailored peptides for biochemical studies, drug discovery, or materials research. It provides a way to incorporate specialized features before cleavage, including detection labels, lipid attachments, reactive handles, and structural elements for cyclization. These modifications expand the range of peptide molecules accessible from solid-phase synthesis.