Intra-amniotic Injection
Intra-amniotic Injection - Related Videos
07:36
Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
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Cited by 20 •
2015
Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.
08:30
Intra-articular Shoulder Injection for Reduction Following Shoulder Dislocation
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2023
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA The anterior shoulder dislocation is one of the most common joint dislocations seen in emergency settings. In anterior shoulder dislocation, the humeral head is displaced out of the glenohumeral joint in front of the scapular glenoid, resulting in a loss of the articulation between the arm and the rest of the shoulder. This can be caused by a fall onto an abducted, extended, and externally...
06:43
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
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Cited by 11 •
2018
We describe a protocol for performing an in utero transplantation (IUT) through intravenous and intra-amniotic routes of injection in the murine model. This protocol can be used to introduce cells, viral vectors, and other substances into the unique immune-tolerant fetal environment.
09:06
Intra-lymph Node Injection of Biodegradable Polymer Particles
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Cited by 45 •
2014
Lymph nodes are the immunological tissues that orchestrate immune response and are a critical target for vaccines. Biomaterials have been employed to better target lymph nodes and to control delivery of antigens or adjuvants. This paper describes a technique combining these ideas to inject biocompatible polymer particles into lymph nodes.
04:20
Intra-uterine Cancer Cell Injection: A Technique to Deliver Cancer Cells for Establishing Intra-peritoneal Ovarian Cancer Mouse Model
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2023
In this video, we demonstrate intra-uterine injection of ovarian cancer cells in a mouse model. This orthotopic murine model helps us to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention and treatment of recurrent ovarian cancer.
FAQs
The amniotic cavity creates several possible exposure routes after delivery: the substance may contact embryonic tissues directly, become distributed through the surrounding fluid, or be swallowed by the developing organism. These routes help explain why one injection can affect more than one developmental site and allow investigators to examine how prenatal exposure reaches tissues.
The surrounding fluid is not merely a passive location. It provides the environment through which the measured material can spread and from which it may reach tissues or be swallowed. Consequently, the substance’s presence in amniotic fluid connects the injection event to questions about tissue contact, internal exposure, and developmental consequences.
Intra-amniotic Injection can be used to examine organ formation, growth, and tissue patterning as separate but related developmental outcomes. By introducing drugs, nutrients, signaling molecules, or potentially harmful compounds, researchers can ask whether prenatal exposure changes a particular aspect of development and use those observations to investigate mechanisms shaping embryonic tissues.
Its value in developmental-toxicity research comes from controlling what substance is introduced and how much is delivered. That defined exposure provides a basis for evaluating potentially harmful compounds alongside developmental outcomes, rather than treating prenatal exposure as an uncontrolled event. The resulting observations can connect a compound with altered formation, growth, or patterning.
A basic workflow begins by selecting a defined substance, determining a measured volume, and using a fine needle to place it into the amniotic cavity. After delivery, investigators consider whether the material contacts tissues, distributes through fluid, or is swallowed. They then relate that exposure to observed developmental changes.
The approach can examine the developmental effects of drugs, nutrients, signaling molecules, and potentially harmful compounds. This range supports questions about both normal developmental regulation and adverse prenatal exposure. Researchers can compare the introduced substance with outcomes involving organ formation, growth, tissue patterning, or broader mechanisms that shape embryonic development.