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JoVE Journal Developmental Biology
Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model
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Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

Article DOI: 10.3791/58047-v • 06:43 min • October 9th, 2018
October 9th, 2018 •
1, 1, 1, 1, 2, 1
1Division of General, Thoracic, and Fetal Surgery, Center for Fetal Research, Children's Hospital of Philadelphia, 2Division of Plastic Reconstructive Surgery, Center for Fetal Research, Children's Hospital of Philadelphia

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Summary
Automatic Translation

We describe a protocol for performing an in utero transplantation (IUT) through intravenous and intra-amniotic routes of injection in the murine model. This protocol can be used to introduce cells, viral vectors, and other substances into the unique immune-tolerant fetal environment.

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Intravenous Intra-amniotic In Utero Transplantation Embryology Gene Therapy Stem Cell Transplantation Murine Model Doctors Nicholas Ahn Barbara Coons Research Fellows Laboratory Surgical Needle Sterile PBS Debris Clearance Reagent Of Interest Experimental Volume And Concentration Micro Injector Injection Calibration Screen Injection Time Adjustment Balance Button Foot Pedal Solution Volume Measurement Pregnant Mouse Abdomen Shaving Heating Pad Application
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