Myh6-dsred-nuc Line
Myh6-dsred-nuc Line - Related Videos
09:34
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
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Cited by 1 •
2016
This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.
08:52
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Cited by 13 •
2015
This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.
02:22
Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells
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2025
This video demonstrates live cell imaging for the analysis of antifungal activity in human neutrophils using fluorescent Aspergillus reporter conidia. Initially, both red and magenta fluorescence are detected within neutrophils, while red fluorescence decreases over time, indicating the degradation of conidia and confirming the antifungal activity of neutrophils.
12:29
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
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Cited by 10 •
2017
Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.
03:41
DFE-Based Affinity Chromatography: A Technique for Purification of 2F5 Monoclonal Antibodies from a Crude Sample Using High Ionic Strength-Based Elution Conditions
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2025
This video describes an affinity chromatography technique for purifying 2F5 monoclonal antibodies using the DsRed-2F5-Epitope or DFE ligands. The purified 2F5 monoclonal antibodies are essential drugs in immunotherapy.
FAQs
Myh6 regulatory sequences link cardiac alpha-myosin heavy chain gene activity to production of nuclear-localized dsRed. As cells activate this cardiomyocyte-associated program, fluorescent protein accumulates in their nuclei, creating a signal tied to regulatory activity rather than simply marking an anatomical region. This relationship helps investigators examine when and where cardiomyocyte differentiation becomes detectable during heart development.
Nuclear localization concentrates the dsRed signal in discrete cellular compartments, making individual labeled cardiomyocytes easier to identify within heart tissue. A nuclear signal supports cell counting and spatial analysis because nuclei provide distinct reference points, even when cardiomyocytes are densely arranged. This feature is particularly useful for comparing cell distributions across embryonic or postnatal samples.
The signal indicates cardiomyocyte-specific gene activity driven by the Myh6 regulatory system. Its presence can therefore help identify cells that have entered a cardiac muscle differentiation program, while changes in labeled-cell number or position can reveal developmental patterns. Interpretation should remain tied to reporter activity, because fluorescence provides a readout of the transgenic regulatory design rather than a complete measurement of every cardiac property.
Researchers can image embryonic or postnatal heart tissue, identify red fluorescent nuclei, and use those signals to visualize, count, and map cardiomyocytes. Spatial analysis can then relate labeled cells to their distribution within developing tissue. This workflow supports examination of cardiac morphogenesis, including how the arrangement and abundance of reporter-positive cells change across developmental stages.
The line is suited to studies of cardiac specification, differentiation, and morphogenesis. Investigators can follow the appearance and distribution of fluorescent cardiomyocyte nuclei while examining how heart muscle cells emerge and become organized. These readouts provide a cellular perspective on developmental changes and can help connect cardiomyocyte patterns with broader changes in embryonic and postnatal heart structure.
In experimental models of disease-related cardiac change, the reporter can help identify and spatially assess cardiomyocytes within heart tissue. Researchers may compare the number or arrangement of fluorescent nuclei between experimental conditions, using the signal to track changes in the heart muscle cell population. The model therefore adds cell-specific context to tissue imaging and developmental or pathological analyses.