Transgenic Fish Embryos
Transgenic Fish Embryos - Related Videos
17:39
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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Cited by 56 •
2007
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
07:45
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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Cited by 4 •
2013
In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.
04:29
Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos
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2025
This video demonstrates the ex vivo culture and imaging of oculomotor slices from transgenic mouse embryos. The embryos are isolated and embedded in agarose. Using a vibratome, slices containing the oculomotor nuclei and eyes are obtained. The slices are maintained in culture and imaged using a fluorescence microscope.
05:40
Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos
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2025
In this video, we demonstrate a procedure to generate a stable zebrafish transgenic line constitutively expressing a fluorescent voltage indicator protein following the co-injection of the Tol2 transposon system comprising the transposase mRNA and Tol2 transposon construct encoding the fluorescent voltage indicator.
09:10
Generation of Transgenic Hydra by Embryo Microinjection
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Cited by 34 •
2014
Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
FAQs
Promoter selection links the introduced DNA sequence to a chosen pattern of gene activity. When the promoter drives the construct, expression can be examined in relation to developmental time and location rather than treated as a uniform event. This makes promoter-controlled embryos useful for asking where and when a gene may influence tissue formation or cell fate.
Genomic integration matters because it places the introduced sequence within the embryo’s genetic material, allowing researchers to examine its activity as development proceeds. Integration alone does not describe the gene’s function; investigators also need an expression readout and developmental observations. Comparing molecular activity with visible changes helps connect a genetic hypothesis to a developmental outcome.
Fluorescent reporter genes convert transgene activity into a visible signal. Researchers can inspect where fluorescence appears and how that pattern changes over time, then relate the signal to forming tissues or changing cell fates. This readout is valuable when the goal is to map gene regulation dynamically in a living embryo and connect expression patterns with developmental processes.
An experimental workflow begins with a DNA construct containing the sequence of interest and a promoter, followed by injection into fertilized fish eggs. Embryos are then observed for reporter expression and developmental features. The procedure combines an early genetic manipulation with observation over time, enabling researchers to associate transgene activity with subsequent tissue formation and other visible outcomes.
These embryos are useful when developmental questions require direct observation in a living organism. Researchers can examine how altered gene activity relates to cell fate, tissue formation, and developmental signaling. Because molecular activity can be compared with visible embryonic changes, the system supports testing genetic hypotheses while preserving the developmental context in which those genes act.
The key connection comes from aligning two observations: where and when the transgene is active, and what developmental features appear as the embryo changes. A reporter provides the activity pattern, while inspection of cell fate and tissue formation provides phenotype-level context. Together, these observations help interpret how gene regulation contributes to developmental change.