Lipid Imaging
Lipid Imaging - Related Videos
08:38
Photoacoustic Tomography to Image Blood and Lipids in the Infrarenal Aorta
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2023
Source: Gurneet S. Sangha and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana Photoacoustic tomography (PAT) is an emerging biomedical imaging modality that utilizes light generated acoustic waves to obtain compositional information from tissue. PAT can be used to image blood and lipid components, which is useful for a wide variety of applications, including cardiovascular and tumor imaging. Currently used imaging techniques have inherent...
10:15
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Cited by 34 •
2015
We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.
11:45
Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
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Cited by 4 •
2012
This article describes a method to visualize formation of an HIV-1 envelope-induced virological synapse on glass supported planar bilayers by total internal reflection fluorescence (TIRF) microscopy. The method can also be combined with immunofluorescence staining to detect activation and redistribution of signaling molecules that occur during HIV-1 envelope-induced virological synapse formation.
07:53
Super-Resolution Imaging of NK Cell Immunological Synapse Formation on a Supported Lipid Bilayer
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2025
This video demonstrates a technique for visualizing the formation of the immunological synapse in natural killer (NK) cells on a supported lipid bilayer (SLB). The SLB is first labeled with a fluorophore-tagged NK cell activating receptor ligand, followed by incubation with NK cells. Subsequently, the cells are stained for polymerized actin and perforin-positive lytic granules. This approach enables the visualization of synapse formation using stimulated emission depletion (STED) microscopy.
03:38
Structure of Lipids
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2020
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
FAQs
The method determines which aspect of a lipid system becomes measurable. Fluorescent probes can visualize selected molecules, label-free optical signals provide measurements without an added imaging label, and mass spectrometry can detect lipid species while mapping their spatial distribution. Comparing these readouts helps distinguish composition, location, and organization rather than treating lipid content as a single measurement.
Lipid location and organization can be connected to physical properties and biological function. Two samples may contain related lipid components yet behave differently if those components occupy different regions or arrangements. For engineered membranes, biomaterials, and delivery systems, spatial information therefore helps explain how molecular composition contributes to structure and performance.
A single measurement shows lipid distribution at one point, whereas time-resolved measurements can follow changes over time. These observations may reveal lipid transport and remodeling, allowing researchers to examine how a system changes rather than only where lipids are initially located. This dynamic information is relevant when evaluating evolving membranes, materials, or engineered delivery systems.
Selection depends on the information the system requires. Fluorescent probes are useful when visualizing selected lipid components, label-free optical signals support direct optical measurement, and mass spectrometry is appropriate when identifying lipid species and their spatial distribution is important. The choice should match whether the design question concerns location, organization, composition, or change over time.
In these systems, imaging can help characterize lipid composition, spatial distribution, and organization. Those measurements connect molecular features with material behavior, supporting the design of more stable lipid nanoparticles and improved drug-delivery systems. The same approach can also help compare engineered formulations by showing how their lipid structure relates to intended performance.
Lipid imaging links molecular composition with physical properties and biological function. In biomaterials and membrane-based systems, this relationship can guide material characterization and model lipid-mediated processes. By mapping how lipids are arranged and how they change, researchers can evaluate design choices, investigate performance, and develop systems that better reproduce relevant biological behavior.