Biotinylated Mirna Mimics
Biotinylated Mirna Mimics - Related Videos
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
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2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
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2023
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
07:01
Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models
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Cited by 2 •
2022
Here, we deliver exogenous artificially synthesized miRNA mimics to the kidney via tail vein injection of a nonviral vector and polyethylenimine nanoparticles in several kidney disease mouse models. This led to significant overexpression of target miRNA in the kidney, resulting in inhibited progression of kidney disease in several mouse models.
FAQs
The guide strand provides the sequence information used by the RNA-induced silencing complex, or RISC, to recognize complementary regions in messenger RNAs. Once this pairing occurs, the associated transcript may undergo translational repression or destabilization. Consequently, the mimic’s regulatory effects depend strongly on which cellular RNAs contain sequences compatible with guide-strand recognition.
Biotin supplies an affinity handle that makes the mimic and its molecular associations easier to detect or capture. After cellular introduction, researchers can use this tag to isolate associated RNAs, proteins, and regulatory complexes. This analytical capability extends the experiment beyond measuring gene regulation, allowing investigators to examine the molecular environment surrounding the mimic.
A synthetic mimic provides a way to restore or model the activity of a mature microRNA in cells. This is useful when researchers want to examine the consequences of a particular regulatory sequence or investigate post-transcriptional control through an introduced reagent. Its use connects sequence-specific miRNA activity with experimentally accessible molecular analysis.
Recognition of a complementary messenger RNA can produce two principal regulatory outcomes described for these mimics: reduced translation or destabilization of the target mRNA. These outcomes link sequence pairing to altered gene expression after RISC loading. Examining which outcome accompanies a given target can help clarify how the modeled microRNA influences post-transcriptional regulation.
A typical workflow begins by introducing the double-stranded mimic into cells, where it can be loaded into RISC and engage complementary messenger RNAs. The biotin tag then supports affinity-based capture or detection of associated molecules. Researchers can examine recovered RNAs, proteins, or regulatory complexes to connect the mimic with its cellular targets and partners.
Biotinylated miRNA mimics support target discovery by enabling isolation of RNAs associated with the introduced regulatory sequence. Analysis of the captured material can also identify proteins and regulatory complexes involved in its activity. These results help researchers map affected pathways and relate molecular associations to broader patterns of post-transcriptional gene regulation in genetics studies.