Haploid-diploid Chimera
Haploid-diploid Chimera - Related Videos
12:40
Making Gynogenetic Diploid Zebrafish by Early Pressure
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Cited by 17 •
2009
This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.
07:54
Production of Haploid Zebrafish Embryos by In Vitro Fertilization
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Cited by 37 •
2014
The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.
07:37
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
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2017
Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.
09:10
Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Cited by 1 •
2013
Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.
09:03
Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
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Cited by 20 •
2010
Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.
FAQs
The key experimental advantage is that haploid and diploid populations can be examined while they develop together. Because each population retains its chromosome complement, researchers can relate differences in cell fate, developmental contribution, or tissue formation to ploidy rather than treating chromosome number as an isolated measurement. This makes the chimera a comparative genetics system.
Maintaining the two ploidy states matters because chromosome number can influence gene expression and genomic imprinting. Comparing these features across the coexisting populations can reveal whether regulatory patterns differ with ploidy. Such analysis connects chromosome complement to developmental behavior without requiring the populations to be studied in separate organisms.
Haploid-diploid chimeras can be used to investigate whether chromosome complement is associated with differences in reproductive cell formation. The same system also supports questions about haploid cell function and developmental potential, helping researchers connect the behavior of haploid populations with broader issues in reproductive biology and genetic development.
Generation begins by combining haploid and diploid embryonic cells or embryos. The resulting developmental system allows both populations to remain present, proliferate, and contribute to developing tissues while retaining their respective ploidy states. Researchers can then examine how each chromosome complement relates to tissue contribution and developmental behavior within the chimera.
Haploid-diploid chimeras are suited to questions about cell fate, development, gene expression, genomic imprinting, and reproductive cell formation. They also provide a way to study haploid cell function and developmental potential in the context of a developing organism. In genetics, this breadth helps link chromosome number with cellular and organismal outcomes.
The model can be incorporated into genome-engineering research alongside questions about different chromosome complements. Its value comes from connecting genetic manipulation with ploidy, haploid cell function, developmental potential, and reproductive biology. This combination broadens the study of engineered genetic effects by considering how they relate to distinct haploid and diploid cell populations.