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Biology

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Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
 

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Article doi: 10.3791/2055
December 22nd, 2010 Usage Statistics

Summary December 22nd, 2010

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

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