Avian Cell Co-culture
Avian Cell Co-culture - Related Videos
14:02
Isolation and Culture of Avian Embryonic Valvular Progenitor Cells
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Cited by 4 •
2010
This article will provide a method for isolating and culturing quail or chicken HH14- valve endocardial cells and HH25 valve cushion mesenchymal cells.
01:31
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2026
Begin with a suspension of avian pathogenic E. coli or APEC in growth media.APEC is a Gram-negative bacterium that infects poultry by entering through the respiratory tract.After breaching the mucosal layer, APEC adhere to epithelial cells, multiply locally, and disrupt the epithelial barrier to enter the bloodstream. The bacteria then spread to multiple organs, causing systemic infection.Add an equal volume of the bacterial suspension to each well of a multi-well plate containing increasing...
08:40
Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
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Cited by 90 •
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12:21
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
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Cited by 13 •
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Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.
12:09
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Cited by 42 •
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A method to preserve, detect and sequence RNA from Avian Influenza Viruses was validated and extended using natural faecal samples from birds. This technique removes the necessity of maintaining a cool chain and handling of infectious viruses and can be applied in a 96-well high-throughput setup.
FAQs
Three communication routes shape the co-culture response: direct cell contact, soluble signaling molecules, and exchanged nutrients or metabolites. Their combined effects can produce coordinated behavior that a single cell population may not show. Separating these routes conceptually helps investigators ask whether a response depends primarily on physical interaction, secreted signals, or changes in the shared chemical environment.
Using two or more avian cell types can reveal interactions that remain invisible in isolated cultures. One population may alter how another responds through contact or shared soluble factors, creating a model of coordinated host behavior rather than a response from one cell type alone. This added biological context is particularly valuable when interpreting immune or infection-related changes.
Pairing immune cells with epithelial or other host cells connects distinct parts of an infection response within one experimental system. Researchers can examine how cellular interactions relate to pathogen entry, replication, inflammatory signaling, and cellular defense. This arrangement places infection-related events alongside immune communication, providing a broader view than studying either cell population separately.
The selected cell types should match the interaction being modeled. For infection research, combining immune cells with epithelial or other host cells can connect cellular defense with responses in potential host tissues. The shared culture environment then permits communication among those populations, allowing investigators to examine coordinated effects under controlled experimental conditions.
A controlled shared environment helps researchers relate observed responses to interactions among the selected avian cell types. Because cells can exchange signals, nutrients, and metabolites, changes may reflect communication across the culture rather than an isolated cellular reaction. This control supports clearer interpretation of coordinated immune, host, or infection-related outcomes.
The method provides a more physiologically relevant setting for evaluating interventions that affect host-pathogen interactions. Researchers can use coordinated avian cell responses to examine how vaccines, antivirals, or immune-modulating treatments influence infection-related processes and cellular defense. Its value lies in testing these effects across interacting cell populations rather than in an isolated culture context.