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Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
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JoVE Journal Medicine
Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model

Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model

DOI:
10.3791/2815-v

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08:40 min

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May 30, 2011

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, ,
1Department of Pathology,Vanderbilt University , 2Departments of Oncology, Surgery and Medical Biophysics,London Regional cancer program

Chapters

  • 00:05Title
  • 01:31Spontaneous Metastasis: Preparing the Eggs
  • 03:13Spontaneous Metastasis: Grafting the Tumor Cells
  • 04:09Experimental Metastasis: Preparing and Injecting Eggs with Tumor Cells
  • 05:19Harvesting the Tumors
  • 06:18Harvesting the Organs
  • 07:14Tumor Cells after Xenografting or Injecting HEp3 Cells
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of human tumor cells to distant organs.

Tags

Quantitative AnalysisCancer MetastasisAvian Embryo ModelPrimary TumorDistant OrgansMetastatic ProcessMetastatic CascadeMetastasis AssaysExperimental AnimalsChick EmbryoChorioallantoic Membrane (CAM)Xenografting Of Tumor CellsImmunodeficientGrowthInvasionAngiogenesisMicroenvironment

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