B Cell Infection
B Cell Infection - Related Videos
06:10
Following Cell-fate in E. coli After Infection by Phage Lambda
0 Views •
Cited by 20 •
2011
This article describes the procedure for preparing a fluorescently-labeled version of bacteriophage lambda, infection of E. coli bacteria, following the infection outcome under the microscope, and analysis of infection results.
07:10
Establishment and Quantification of De Novo Lytic Infection by Cell-free Kaposi's Sarcoma-Associated Herpesvirus
0 Views •
2025
This protocol describes an in vitro system to model de novo lytic infection of Kaposi's sarcoma-associated herpesvirus using BAC16-derived virions. The method enables investigation of early viral replication and dissemination. Infectious virus production is quantified by GFP-based infectious units assays and real-time PCR of encapsidated viral genomes.
13:54
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
0 Views •
Cited by 2 •
2013
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
11:18
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
0 Views •
Cited by 234 •
2012
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
12:38
4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
0 Views •
Cited by 19 •
2013
Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.
FAQs
Pathogens begin by binding receptors on the B-cell surface, which provides the entry point for infection. After attachment, they can enter the cell and use its cellular machinery to replicate or establish persistence. Examining receptor interactions helps researchers identify how host cells become susceptible and how early infection may shape later immune responses.
Once inside a B cell, an infectious agent may modify signaling pathways that normally regulate immune activity, antigen presentation, or survival. These changes can affect how the cell communicates with other immune components and whether it remains viable. Studying these effects helps explain how infection produces immune dysfunction or allows infected cells to persist.
Changes in antibody production can weaken or redirect humoral immune responses, while altered antigen presentation may affect how immune information is displayed to other cells. Together, these disruptions can help an infection evade adaptive immunity. Measuring both functions gives a broader view of how B-cell infection affects immune regulation beyond pathogen replication alone.
Experimental models can follow the major sequence from pathogen binding to B-cell entry, use of cellular machinery, replication, or persistence. They can also examine changes in signaling, antigen presentation, survival, and antibody production. Comparing these outcomes helps researchers connect an infectious agent’s cellular behavior with the resulting immune effects.
These models are useful for investigating host-pathogen interactions and antiviral responses, especially when researchers need to connect cellular infection with adaptive immune dysfunction. They also support vaccine-design studies by revealing how infection affects B-cell functions. The resulting information can guide questions about preventing persistence or restoring effective immune regulation.
Persistent or dysregulated infection of B cells may contribute to chronic disease or lymphoproliferation, making these cells relevant targets for investigation. Research can examine whether infected or altered B cells should be selectively targeted and how their immune effects develop. This context links cellular infection studies with therapies aimed at infected or dysregulated B cells.