Bioluminescent Pseudomonas
Bioluminescent Pseudomonas - Related Videos
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
05:06
Bioluminescent Bacterial Imaging In Vivo
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Cited by 9 •
2012
This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging.
03:07
Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse
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2025
Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (2017)This video demonstrates the bioluminescence imaging of neuroinflammation in transgenic mice, showcasing luciferase-luciferin reactions in activated astrocytes. The protocol enables non-invasive quantification of astrocytic activation, aiding studies on CNS inflammation and therapeutic efficacy.
02:46
A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos
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2025
The video demonstrates the phage therapy technique targeting Pseudomonas aeruginosa infection in cystic fibrosis zebrafish embryos. Phage therapy effectively diminishes bacterial load and mitigates proinflammatory immune responses in CF zebrafish embryos infected with bacteria, leading to reduced CF embryo lethality.
02:02
Bioluminescence Imaging of Bacterial Infections in Mice
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2026
Source: de Vries, C. R., et al., A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020).This video demonstrates a bioluminescent imaging technique to monitor bacterial infection progression in a live mouse model. Pathogenic bacteria engineered with the luxABCDE reporter system emit light at the infection site, allowing visualization of metabolically active bacteria. By imaging the mouse over time, researchers can non-invasively track infection...
FAQs
Light production depends on a luciferase-mediated biochemical reaction that uses a reduced flavin substrate, a long-chain aldehyde, and oxygen. These components provide the chemical basis for visible emission, allowing bacterial activity to be detected without relying solely on endpoint measurements. In infection studies, the resulting signal helps reveal where microbial growth or persistence occurs over time.
In many strains, quorum-sensing signals coordinate expression of the luminescence genes as cell density changes. This links the strength or presence of light production to population-level communication rather than to isolated cells alone. Consequently, researchers can use luminescence patterns to examine how bacterial abundance and collective behavior develop during colonization or infection.
Cell density matters because it can influence quorum-sensing activity and, in turn, the expression of luminescence genes. A changing signal may therefore reflect shifts in the bacterial population or its coordinated behavior during an infection process. Considering density-related regulation helps investigators interpret light patterns as dynamic biological information rather than as a static label for bacterial presence.
Researchers monitor the emitted light over time to follow microbial colonization and infection progression. This approach can show temporal changes in bacterial behavior while also revealing spatial patterns of host-pathogen interaction. The information supports studies of how infection develops in relation to host responses, providing a continuous view that complements endpoint sampling.
Tracking luminescent bacteria allows investigators to relate microbial behavior to host immune responses during infection. Because the signal can be observed as processes unfold, researchers can examine changing relationships between bacterial colonization and immune activity rather than relying only on a final measurement. This makes the method useful for studying host-pathogen interactions in immunology and infection research.
Researchers can use changes in bacterial light emission to monitor the effect of antimicrobial treatments during an ongoing infection study. Measurements over time may indicate how treatment influences microbial growth, persistence, or progression, while spatial patterns can show where effects occur. This real-time perspective may reduce the need for repeated endpoint sampling and improve assessment of treatment responses.