Carboxymethylcellulose

Carboxymethylcellulose is a water-soluble cellulose derivative in which carboxymethyl groups replace some hydroxyl groups, making it useful as a polymeric thickener, stabilizer, and biomaterial. In aqueous environments, its charged polysaccharide chains hydrate, expand, and increase viscosity, while their mucoadhesive properties can promote retention on biological surfaces and influence diffusion. In immunology and infection research, carboxymethylcellulose supports drug-delivery formulations, wound dressings, and hydrogels designed to control the release of antimicrobial or anti-inflammatory agents. Its tunable solubility, viscosity, and interactions with cells and tissues make it valuable for studying localized therapies and improving treatment performance.

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JoVE EoE - Neurophysiology

Preparing a Rat Model to Assess Visual Pathway Integrity Using an Electrophysiology Setup

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2025

This video demonstrates the preparation of a rat model to study retinal health and visual pathway integrity. Visual evoked potential and electroretinography reference and active electrodes are connected to the rat, followed by aligning the platform with the rat in front of the Ganzfeld bowl for even retinal illumination and closing the Faraday cage to avoid external interference.

An Assay to Measure the Neutralizing Antibody Titer Specific for Respiratory Syncytial Virus

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2025

This video demonstrates an assay for measuring neutralizing antibodies against respiratory syncytial virus (RSV). Pre-incubation with serum containing RSV-neutralizing antibodies neutralizes the virus particles, reducing cellular infection and plaque formation. Virus-infected cells are then subjected to fixation, blocking, and fluorophore-conjugated antibody labeling for plaque visualization. This process enables the identification of the highest serum dilution that neutralizes fifty percent of...

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats

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Cited by 13 •

2016

This protocol describes simultaneous measurement of electroretinogram and visual evoked potentials in anesthetized rats.

Antibody-Mediated Neutralization of Coronavirus Spike Protein-Expressing Pseudoviruses Using Patient Serum

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2026

Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the antibody-mediated neutralization of pseudoviruses carrying coronavirus spike proteins using patient serum, studied through high-throughput fluorescent imaging of infected epithelial cell monolayers.

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

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2021

The protocol described here outlines a fast and effective method for measuring neutralizing antibodies against the SARS-CoV-2 spike protein by evaluating the ability of convalescent serum samples to inhibit infection by an enhanced green fluorescent protein-labeled vesicular stomatitis virus pseudotyped with spike glycoprotein.

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