Feed Arteriole
Feed Arteriole - Related Videos
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
03:41
Preparing Cerebral Endothelial Tubes from Mouse Parenchymal Arterioles
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2025
This video demonstrates a method for isolating the endothelial tubes from mouse cerebral parenchyma. Through a combination of enzymatic digestion and mechanical dissociation, the process yields an intact tube with endothelial cells.
07:35
Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
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Cited by 12 •
2018
Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
01:16
Arteries and Arterioles
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2024
Arteries, the vasculature responsible for transporting blood from the heart, possess robust walls capable of enduring the elevated pressures exerted by the heartbeat. Arteries near the heart are especially thick-walled and enriched with elastic fibers across their three tunics, classifying them as elastic or conducting arteries. These arteries, usually with a diameter exceeding 10 mm, are characterized by their ability to dilate in response to the blood pumped from the heart's ventricles and...
02:13
Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster
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2023
This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).
FAQs
Changes in smooth-muscle tone modify vessel diameter, which changes resistance to flow. Vasoconstriction narrows the vessel and reduces the amount of blood entering downstream capillaries, whereas vasodilation widens it and increases entry. This provides a local mechanism for shifting perfusion as tissue demands or inflammatory conditions change.
During infection, altered tone can redistribute blood entering a local capillary bed. Greater constriction raises resistance and limits delivery, while relaxation lowers resistance and permits more perfusion. Because blood carries oxygen, nutrients, immune cells, antibodies, and inflammatory mediators, either direction can change the conditions under which host defense and tissue inflammation occur.
Diameter determines how much blood reaches the downstream capillaries, so it affects access to several circulating components at once. Increased entry can increase delivery of immune cells, antibodies, and inflammatory mediators to affected tissue, while reduced entry limits that supply. The same adjustment also changes oxygen and nutrient delivery, linking perfusion with local defense.
Changes in tone are linked with local inflammation, vascular leakage, and tissue injury. Examining constriction or dilation alongside these outcomes helps determine whether altered perfusion is occurring with inflammatory changes and leakage. The vessel is therefore a useful point for connecting microcirculatory regulation to tissue effects during infection, without treating blood flow as separate from inflammation.
A focused assessment should relate vascular smooth-muscle state to vessel diameter, resistance, blood pressure, and the amount of blood entering capillaries. In an immunology or infection context, these variables can then be considered alongside delivery of oxygen, nutrients, immune cells, antibodies, and inflammatory mediators. This links vascular change to functional tissue consequences.
They become relevant when the question concerns how local blood flow affects host defense or inflammatory damage. Investigators can use the vessel as a framework for relating perfusion changes to delivery of immune cells, antibodies, and inflammatory mediators, while also considering oxygen, nutrients, vascular leakage, and tissue injury. This connects microcirculation with both defense and damage.