7,142 Views
•
07:35 min
•
May 08, 2018
DOI:
Summary
Automatically generated
Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
Related Videos
07:32
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
Related Videos
14386 Views
10:14
Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
Related Videos
14992 Views
09:33
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
Related Videos
7152 Views
08:45
Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples
Related Videos
4169 Views
09:28
Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
Related Videos
8397 Views
12:28
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
Related Videos
3539 Views
07:35
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Related Videos
2111 Views
04:04
Measuring Retinal Vessel Diameter from Mouse Fluorescent Angiography Images
Related Videos
578 Views
12:42
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
Related Videos
10274 Views
10:19
Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
Related Videos
9444 Views
Read Article
Cite this Article
Gericke, A., Goloborodko, E., Pfeiffer, N., Manicam, C. Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo. J. Vis. Exp. (135), e56199, doi:10.3791/56199 (2018).
Copy