Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
Gericke, A., Goloborodko, E., Pfeiffer, N., Manicam, C. Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo. J. Vis. Exp. (135), e56199, doi:10.3791/56199 (2018).