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Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
 

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

Article DOI: 10.3791/56199-v 07:35 min May 8th, 2018
May 8th, 2018

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Summary

Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.

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Keywords: Retinal Arterioles Retina Preparation Mouse Retina Retinal Blood Flow Video Microscopy Retinal Blood Vessel Reactivity Ophthalmic Artery Orbital Tissue Krebs-Henseleit Buffer Vannas Scissors Precision Tweezers Cornea Choroid Sclera
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