Het-1a Cells
Het-1a Cells - Related Videos
09:51
Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
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Cited by 2 •
2017
Here we present a protocol to image cells expressing green fluorescent protein-tagged angiotensin type 1a receptors during endocytosis initiated by angiotensin II treatment. This technique includes labeling lysosomes with a second fluorescent marker, and then utilizing software to analyze the co-localization of receptor and lysosome in three dimensions over time.
08:22
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
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2020
Here we describe a method for retroviral overexpression and adoptive transfer of murine B-1a cells to examine in vivo B-1a cell migration and localization. This protocol can be extended for diverse downstream functional assays including quantification of donor B-1a cell localization or analysis of donor cell-derived secreted factors post-adoptive transfer.
05:18
Establishing a Primary Dorsal Root Ganglia Cell Culture from a Rat Spinal Column
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2025
This video demonstrates a technique for isolating and culturing dorsal root ganglia (DRG) cells from the rat spinal column. DRGs excised from the spinal column are treated with enzymes and mechanically dissociated to obtain a single-cell suspension. The cells are cultured in a medium containing inhibitors and growth factors that limit glial cell proliferation and promote neuronal growth, respectively.
07:07
Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells
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Cited by 21 •
2015
Stem cell-derived retinal pigment epithelium (RPE) cells may be used for multiple applications including cell-based therapies for retinal degeneration, disease modeling, and drug studies. Here we present a simple protocol for reproducibly deriving RPE from stem cells.
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Cited by 24 •
2014
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
FAQs
Het-1A cells proliferate continuously because recombinant simian virus 40 large T antigen interferes with key cell-cycle control pathways. This supports sustained maintenance under laboratory conditions and keeps an epithelial population available for repeated, controlled studies. The resulting culture system is useful when investigators need to examine infection or inflammation across comparable experimental settings.
A continuous epithelial monolayer provides a tissue-relevant surface for examining microbial contact, epithelial injury, and inflammation-associated signaling. Because the cells can be maintained in a reproducible culture format, investigators can compare how infection-related conditions affect the same general epithelial barrier context. This supports controlled analysis of host-microbe interactions at the esophageal surface.
These cells allow investigators to examine cytokine signaling alongside pathogen attachment, invasion, and epithelial injury. That combination matters because infection-related damage and inflammatory communication can be studied within the same epithelial model rather than as isolated phenomena. The resulting observations help characterize how microbes interact with host tissue and how the epithelium responds.
By examining pathogen attachment and invasion as related but distinct outcomes, researchers can ask whether a microbe primarily associates with the epithelial surface, penetrates epithelial tissue, or produces evidence of both behaviors. This distinction helps connect microbial behavior with epithelial injury and host responses, strengthening mechanistic studies of infection in an esophageal epithelial context.
Their reproducible growth allows investigators to examine how potential antimicrobial or anti-inflammatory interventions affect microbe-host interactions and epithelial responses. Studies can focus on outcomes such as pathogen attachment or invasion, epithelial injury, and cytokine signaling. This makes the model useful for comparing intervention effects under controlled laboratory conditions relevant to esophageal disease mechanisms.
The model connects microbial behavior with epithelial host defense and inflammation in an esophageal setting. Investigators can use it to study how pathogens interact with mucosal tissue, how epithelial injury develops, and how cytokine signaling contributes to the response. These observations provide experimental context for understanding esophageal disease mechanisms and host-microbe interactions.