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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Neuroscience
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JoVE Journal Neuroscience
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

DOI:
10.3791/51609-v

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10:33 min

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May 22, 2014

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, , ,
1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Chapters

  • 00:05Title
  • 01:38Plating of Human Pluripotent Stem Cells for Differentiation
  • 03:08Induction of Differentiation of Neural Crest Cells
  • 04:54Replating of Neural Crest Cells for Specification
  • 07:37Replating of FACS Sorted Cells
  • 08:56Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.

Tags

Feeder-freeNeural Crest Progenitor CellsHuman Pluripotent Stem CellsIn Vitro DifferentiationEmbryonic DevelopmentPeripheral Nervous SystemMelanocytesMesenchymal CellsRegenerative MedicineDisease Modeling

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