Microtiter Dish Assay
Microtiter Dish Assay - Related Videos
03:57
Microtiter Dish Biofilm Formation Assay
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Cited by 2243 •
2011
The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.
06:30
Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography
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Cited by 3 •
2019
The growth of radiolabeled bacterial cultures in microtiter dishes facilitates high throughput sampling that allows multiple technical and biological replicate assays of nucleotide pool abundance, including that of (p)ppGpp. The effects of growth transitions provoked by sources of physiological stress as well as recovery from stress can be monitored.
12:23
Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
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Cited by 149 •
2011
In this protocol we present a method to measure Caenorhabditis elegans lifespan in 96 well microtiter plates.
05:16
A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic
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2025
This video demonstrates a microtiter plate-based method for determining the Minimum Inhibitory Concentration (MIC) of test antibiotics against bacteria. Upon adding a suspension of the bacterial pathogen Pseudomonas aeruginosa to a microtiter plate containing serial dilutions of a test antibiotic, and an inhibitor of the production of pyomelanin by the bacteria, the optical density of the wells is measured to compute the minimum inhibitory concentration of the antibiotic, and the impact of the...
08:10
Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
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Cited by 43 •
2015
C. elegans is an attractive model organism to study signal transduction pathways involved in oxidative stress resistance. Here we provide a protocol to measure oxidative stress resistance of C. elegans animals in liquid phase, using several oxidizing agents in 96 well plates.
FAQs
The measured signal reflects microbial material that remains attached to the treated well surface after incubation and washing. Crystal violet can stain this retained biomass, and absorbance provides a quantitative readout. Comparing absorbance values across wells allows researchers to assess differences in biofilm formation or related infection phenotypes among strains or experimental conditions.
Washing removes cells and material that did not remain attached to the well surface. This focuses the subsequent staining and absorbance measurement on retained biomass rather than the entire microbial culture. Consistent washing is therefore important when comparing wells, because differences in removal can influence the apparent level of surface-associated growth.
Treatment conditions can alter how strongly microbial cells attach to surfaces and whether they develop a detectable biofilm. Using the same plate format and comparing treated wells with different strains or experimental conditions helps reveal these effects. The resulting absorbance measurements support standardized comparisons of microbial behavior and antimicrobial activity across parallel samples.
A typical workflow places microbial cultures into treated wells and incubates them so cells can attach and form biofilms. The wells are then washed to remove unattached material, stained with crystal violet, and assessed by absorbance. Applying the same sequence across many wells enables parallel measurement of retained biomass under multiple experimental conditions.
Researchers can use the assay to compare how experimental treatments affect microbial surface-associated growth. Cultures are incubated in treated wells, and the retained biomass remaining after washing is stained and quantified. Differences between conditions provide a scalable way to screen antimicrobial activity and identify treatments associated with reduced or altered biofilm formation.
In infection research, surface attachment and biofilm formation represent measurable microbial behaviors that can vary among strains or experimental conditions. A microtiter format lets investigators examine these differences across many samples using low volumes and standardized measurements. The resulting comparisons can help characterize infection-related phenotypes and evaluate treatments relevant to infectious disease studies.